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AB226272

Recombinant E. coli Beta-lactamase CTX-M-1 protein (His tag)

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Recombinant E. coli Beta-lactamase CTX-M-1 protein (His tag) is a Escherichia coli Full Length protein, in the 29 to 291 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

men1, bla, Beta-lactamase CTX-M-1, Beta-lactamase MEN-1, Cefotaximase 1

3 이미지
Mass Spectrometry - Recombinant E. coli Beta-lactamase CTX-M-1 protein (His tag) (AB226272)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant E. coli Beta-lactamase CTX-M-1 protein (His tag) (AB226272)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab226272 could indicate that this peptide derived from E.coli-expressed Escherichia coli Beta-lactamase CTX-M-1.

Mass Spectrometry - Recombinant E. coli Beta-lactamase CTX-M-1 protein (His tag) (AB226272)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant E. coli Beta-lactamase CTX-M-1 protein (His tag) (AB226272)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab226272 could indicate that this peptide derived from E.coli-expressed Escherichia coli Beta-lactamase CTX-M-1.

SDS-PAGE - Recombinant E. coli Beta-lactamase CTX-M-1 protein (His tag) (AB226272)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant E. coli Beta-lactamase CTX-M-1 protein (His tag) (AB226272)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226272 with 5% enrichment gel and 15% separation gel.

주요 정보

Purity

>90% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Escherichia coli

보관 버퍼

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB116027

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB116027)

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  • 2
Proteins & Peptides

AB104926

Recombinant E. coli Beta-lactamase protein (His tag N-Terminus)

SDS-PAGE - Recombinant E. coli Beta-lactamase protein (His tag N-Terminus) (AB104926)

  • 0
  • 0
Assay Kits

AB197008

Beta Lactamase Activity Assay Kit (Colorimetric)

Functional Studies - Beta Lactamase Activity Assay Kit (Colorimetric) (AB197008)

  • 0
  • 0
Proteins & Peptides

AB273530

Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag)

ELISA - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

  • 0
  • 0
Proteins & Peptides

AB152320

Recombinant Human Cytochrome P450 17A1/CYP17A1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Cytochrome P450 17A1/CYP17A1 protein (GST tag N-Terminus) (AB152320)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"QTADVQQKLAELERQSGGRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKKSDLVNYNPIAEKHVDGTMSLAELSAAALQYSDNVAMNKLISHVGGPASVTAFARQLGDETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNTTGAASIQAGLPASWVVGDKTGSGDYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESRRDVLASAAKIVTNGL","proteinLength":"Full Length","predictedMolecularWeight":"32.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":291,"aminoAcidStart":29,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P28585","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The CTX-M-1 beta-lactamase also known as CTX-M or CTX-M protein is a member of the class A beta-lactamases specifically extended spectrum beta-lactamases (ESBLs). This enzyme has a molecular weight of approximately 29 kDa. CTX-M-1 is primarily expressed in gram-negative bacteria particularly Escherichia coli frequently referred to as E. coli beta lactamase. Its primary function is to hydrolyze beta-lactam antibiotics including penicillins and cephalosporins rendering them inactive.
Biological function summary

The CTX-M-1 enzyme plays an important role in antibiotic resistance. It hydrolyzes the beta-lactam ring structure of antibiotics which allows bacteria to survive in environments with these antimicrobials. CTX-M-1 functions as a single protein unit and does not form part of a larger complex. This resistance mechanism contributes to the increased survival of E. coli and other bacteria in clinical settings where antibiotics are often used.

Pathways

CTX-M-1 beta lactamase impacts the antibiotic resistance pathway. It directly contributes to the breakdown of beta-lactam antibiotics which is an important step in resistance pathways. CTX-M-1 often interacts with other antibiotic resistance proteins like TEM and SHV beta-lactamases which also hydrolyze related compounds. These interactions complicate treatment strategies as the proteins together enhance a bacteria's resistance profile.

CTX-M-1 beta lactamase plays a significant role in urinary tract infections (UTIs) and sepsis caused by drug-resistant E. coli. These infections are challenging to treat due to the presence of CTX-M-1 rendering many frontline antibiotics ineffective. The enzyme's activity relates to other proteins such as Klebsiella pneumoniae carbapenemase (KPC) which shares a similar antibiotic resistance role contributing to multidrug-resistant infections.
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일반 정보

기능

Extended-spectrum beta-lactamase (ESBL) which confers resistance to penicillins, as well as first, second and third-generation cephalosporins (PubMed : 15155242, PubMed : 1633193, PubMed : 18281307, PubMed : 21730121). Has cefotaxime-hydrolyzing activity (PubMed : 1633193, PubMed : 18281307, PubMed : 21730121). Inactive against the cephamycin antibiotic, cefoxitin, or against the carbapenem, imipenem (PubMed : 1633193, PubMed : 18281307).

서열 유사성

Belongs to the class-A beta-lactamase family.

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제품 프로토콜

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타겟 정보

Extended-spectrum beta-lactamase (ESBL) which confers resistance to penicillins, as well as first, second and third-generation cephalosporins (PubMed : 15155242, PubMed : 1633193, PubMed : 18281307, PubMed : 21730121). Has cefotaxime-hydrolyzing activity (PubMed : 1633193, PubMed : 18281307, PubMed : 21730121). Inactive against the cephamycin antibiotic, cefoxitin, or against the carbapenem, imipenem (PubMed : 1633193, PubMed : 18281307).
See full target information bla
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Product promise

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