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AB236935

Recombinant E. coli E. coli heat labile toxin A protein (Tagged)

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Recombinant E. coli E. coli heat labile toxin A protein (Tagged) is a Escherichia coli Full Length protein, in the 19 to 258 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

대체 명칭 보기

ltpA, eltA, Heat-labile enterotoxin A chain, LTP-A, Heat labile enterotoxin A subunit, LTP A, eltA

1 이미지
SDS-PAGE - Recombinant E. coli E. coli heat labile toxin A protein (Tagged) (AB236935)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant E. coli E. coli heat labile toxin A protein (Tagged) (AB236935)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel analysis of ab236935.

주요 정보

Purity

>85% SDS-PAGE

발현 시스템

Escherichia coli

Tags

10x His tag N-Terminus Myc tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Escherichia coli

보관 버퍼

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

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  • 0
Primary Antibodies

AB188541

Anti-LT subunit (A + B) antibody

Immunoprecipitation - Anti-LT subunit (A + B) antibody (AB188541)

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Proteins & Peptides

AB236164

Recombinant Pseudomonas aeruginosa ompF protein (His tag)

SDS-PAGE - Recombinant Pseudomonas aeruginosa ompF protein (His tag) (AB236164)

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Proteins & Peptides

AB48737

Recombinant Human Retinoid X Receptor alpha/RXRA protein (Tag Free)

SDS-PAGE - Recombinant Human Retinoid X Receptor alpha/RXRA protein (Tag Free) (AB48737)

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  • 0
Proteins & Peptides

AB167861

Recombinant Human FAM84A protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human FAM84A protein (His tag N-Terminus) (AB167861)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"NGDRLYRADSRPPDEIKRSGGLMPRGHNEYFDRGTQMNINLYDHARGTQTGFVRYDDGYVSTSLSLRSAHLAGQSILSGYSTYYIYVIATAPNMFNVNDVLGVYSPHPYEQEVSALGGIPYSQIYGWYRVNFGVIDERLHRNREYRDRYYRNLNIAPAEDGYRLAGFPPDHQAWREEPWIHHAPQGCGNSSRTITGDTCNEETQNLSTIYLREYQSKVKRQIFSDYQSEVDIYNRIRDEL","proteinLength":"Full Length","predictedMolecularWeight":"32.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":258,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P06717","tags":[{"tag":"10x His","terminus":"N-Terminus"},{"tag":"Myc","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

E. coli heat labile toxin A also known as LT-A is one component of the heat-labile enterotoxin produced by enterotoxigenic Escherichia coli (ETEC). LT-A has a molecular mass of approximately 27 kDa. This toxin consists of two subunits: the enzymatically active A subunit and the pentameric B subunit. Primarily LT-A gets expressed in the intestinal lumen where ETEC colonizes. LT-A is responsible for the ADP-ribosylation of the alpha subunit of G proteins leading to sustained activation of adenylate cyclase.
Biological function summary

E. coli heat labile toxin A plays a significant role in causing traveler's diarrhea by disrupting water and electrolyte absorption in enterocytes. It is part of the ab5 toxin family where it forms a complex with the B subunits to exert its toxic effects. The disruption occurs when the catalytic A subunit enters the host cell after the B subunit binds to the GM1 gangliosides on the cell membrane. The continuous activation of adenylate cyclase increases intracellular cyclic AMP (cAMP) levels leading to chloride ion and water secretion into the intestinal lumen.

Pathways

The elevation of cAMP levels driven by E. coli heat labile toxin A activates the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel pathway. This process closely relates to the protein Gs alpha which mediates the signal transduction process by activating adenylate cyclase. Another important pathway involves signal crosstalk with the PKC and PKA pathways influencing the regulation of ion transport and fluid secretion in epithelial cells.

E. coli heat labile toxin A links with conditions such as cholera-like diarrhea and traveler's diarrhea. Both of these conditions result from excessive fluid loss due to enhanced chloride ion secretion. The toxin-related pathway involves interactions with proteins such as Gs alpha and CFTR critical components in pathogenesis. Because of the effects on chloride ion transport researchers also study LT-A for its role in understanding cystic fibrosis symptoms and potential treatments.
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일반 정보

기능

The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.

서열 유사성

Belongs to the enterotoxin A family.

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제품 프로토콜

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타겟 정보

The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.
See full target information eltA
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Product promise

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