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AB119740

Recombinant E. coli GFP protein (His tag N-Terminus)

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(4 제품이 사용된 논문 )

Recombinant E. coli GFP protein (His tag N-Terminus) is a Aequorea victoria Full Length protein, in the 1 to 238 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, WB.
1 이미지
SDS-PAGE - Recombinant E. coli GFP protein (His tag N-Terminus) (AB119740)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant E. coli GFP protein (His tag N-Terminus) (AB119740)

The purity of ab119740 was determined to be >80% by densitometry.

주요 정보

Purity

>80% SDS-PAGE

The purity was determined to be 80% by densitometry

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P42212-1

Animal free

No

Carrier free

No

Species

Aequorea victoria

보관 버퍼

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.71% Sodium phosphate, 0.005% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

  • 0
  • 0
Proteins & Peptides

AB199750

Recombinant mCherry protein (His tag)

  • 4
  • 1
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Assay Kits

AB171581

GFP ELISA Kit

SimpleStep

Sandwich ELISA - GFP ELISA Kit (AB171581)

  • 5
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"39 kDa","actualMolecularWeight":null,"aminoAcidEnd":238,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P42212","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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일반 정보

기능

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

서열 유사성

Belongs to the GFP family.

Post-translational modifications

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

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제품 프로토콜

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타겟 정보

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP

대체 명칭 보기

Green fluorescent protein, GFP
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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biomaterials 288:121671 PubMed35953331

2022

Debulking different Corona (SARS-CoV-2 delta, omicron, OC43) and Influenza (H1N1, H3N2) virus strains by plant viral trap proteins in chewing gums to decrease infection and transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Henry Daniell,Smruti K Nair,Hancheng Guan,Yuwei Guo,Rachel J Kulchar,Marcelo D T Torres,Md Shahed-Al-Mahmud,Geetanjali Wakade,Yo-Min Liu,Andrew D Marques,Jevon Graham-Wooten,Wan Zhou,Ping Wang,Sudheer K Molugu,William R de Araujo,Cesar de la Fuente-Nunez,Che Ma,William R Short,Pablo Tebas,Kenneth B Margulies,Frederic D Bushman,Francis K Mante,Robert P Ricciardi,Ronald G Collman,Mark S Wolff

MicrobiologyOpen 10:e1239 PubMed34713604

2021

Characterization of endogenous promoters of GapC1 and GS for recombinant protein expression in Phaeodactylum tricornutum.

Applications

Unspecified application

Species

Unspecified reactive species

Erdenedolgor Erdene-Ochir,Bok-Kyu Shin,Md Nazmul Huda,Eun Ha Lee,Dae-Geun Song,Choonkyun Jung,Cheol-Ho Pan

BMC plant biology 21:208 PubMed33952221

2021

Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus.

Applications

Unspecified application

Species

Unspecified reactive species

Wenshuang Xie,Dee Marie Marty,Junhuan Xu,Nitika Khatri,Kristen Willie,Wanderson Bucker Moraes,Lucy R Stewart

Scientific reports 9:9941 PubMed31289300

2019

Identification and characterisation of the novel endogenous promoter HASP1 and its signal peptide from Phaeodactylum tricornutum.

Applications

Unspecified application

Species

Unspecified reactive species

Erdenedolgor Erdene-Ochir,Bok-Kyu Shin,Byeori Kwon,Choonkyun Jung,Cheol-Ho Pan
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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