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AB235854

Recombinant E. coli Glycerol 3 Phosphate Dehydrogenase protein (His tag)

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Recombinant E. coli Glycerol 3 Phosphate Dehydrogenase protein (His tag) is a Escherichia coli K-12 Full Length protein, in the 1 to 501 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

대체 명칭 보기

glyD, b3426, JW3389, glpD, Aerobic glycerol-3-phosphate dehydrogenase

1 이미지
SDS-PAGE - Recombinant E. coli Glycerol 3 Phosphate Dehydrogenase protein (His tag) (AB235854)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant E. coli Glycerol 3 Phosphate Dehydrogenase protein (His tag) (AB235854)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel using ab235854.

주요 정보

Purity

>90% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Escherichia coli K-12

보관 버퍼

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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보완 제품

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Recombinant Human SBCAD protein (His tag N-Terminus)

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Assay Kits

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Proteins & Peptides

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Recombinant Human LTBP1 protein (GST tag N-Terminus)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSASSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIRIGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVVRKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGMHLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYKGDPKAVKIEESEINYLLNVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDYTLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIEGDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAELKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQRLSLAS","proteinLength":"Full Length","predictedMolecularWeight":"60.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":501,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P13035","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Glycerol 3-Phosphate Dehydrogenase (G3PDH) also known as G3P dehydrogenase and glycerol-3-phosphate dehydrogenase plays a mechanical role in the reversible redox conversion of dihydroxyacetone phosphate (DHAP) to glycerol 3-phosphate. The enzyme typically weighs around 40-50 kDa although the exact mass depends on isoform. G3PDH is expressed in various tissues with significant presence in the brain liver and skeletal muscle reflecting its involvement in energy metabolism. The mitochondrial and cytosolic forms add to its versatility in cellular compartments.
Biological function summary

This enzyme is critical for lipid and carbohydrate metabolism engaging extensively in energy homeostasis. G3PDH exists as part of a complex to facilitate its role in mitochondrial electron transport. It acts as a shuttle transferring electrons from cytosolic NADH into the mitochondria. This electron transport is essential for synthesizing ATP thereby linking metabolic pathways within cells.

Pathways

Glycerol-3-phosphate dehydrogenase participates actively in the glycerol phosphate shuttle and lipid biosynthesis pathways. It pairs functionally with its partner the mitochondrial glycerol 3-phosphate dehydrogenase (mG3PDH) to support efficient energy production and lipid storage. Additionally this enzyme relates closely to proteins like NADH dehydrogenase playing a role in electron transport chains in mitochondria critical for bioenergetics.

G3PDH has connections to metabolic conditions such as obesity and diabetes. Impaired function of this enzyme can lead to disruptions in metabolic pathways which can contribute to these diseases. It also holds significance in cancer metabolism with altered expression of G3PDH seen in various tumors. The enzyme's relation to proteins like mG3PDH highlights its role in metabolic syndrome and associated pathologies.
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일반 정보

기능

Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses molecular oxygen or nitrate as electron acceptor.

서열 유사성

Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.

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제품 프로토콜

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타겟 정보

Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses molecular oxygen or nitrate as electron acceptor.
See full target information glpD
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Product promise

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