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AB63839

Recombinant E. coli GrpE protein (Tag Free)

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Recombinant E. coli GrpE protein (Tag Free) is a Escherichia coli K-12 Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.
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SDS-PAGE - Recombinant E. coli GrpE protein (Tag Free) (AB63839)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant E. coli GrpE protein (Tag Free) (AB63839)

3μg protein in 15% SDS-PAGE.

주요 정보

Purity

>90% SDS-PAGE

ab63839 was overexpressed in E.coli and purified by using the conventional column chromatography techniques.

발현 시스템

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P09372-1

Animal free

No

Carrier free

No

Species

Escherichia coli K-12

보관 버퍼

pH: 8 Constituents: 0.58% Sodium chloride, 0.242% Tris

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB171523

TMB ELISA Substrate (High Sensitivity)

ELISA - TMB ELISA Substrate (High Sensitivity) (AB171523)

  • 0
  • 0
Assay Kits

AB102504

Coenzyme A Assay Kit

Functional Studies - Coenzyme A Assay Kit (AB102504)

  • 0
  • 0
Secondary Antibodies

AB205719

Goat Anti-Mouse IgG H&L (HRP)

Western blot - Goat Anti-Mouse IgG H&L (HRP) (AB205719)

  • 5
  • 4
Proteins & Peptides

AB7403

Streptavidin (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin (HRP) (AB7403)

  • 5
  • 7
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKIANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSLDRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMVESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P09372","tags":[]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The GrpE protein also known simply as GrpE functions as a molecular chaperone and co-chaperone in bacteria assisting in protein folding and repair by helping the Hsp70 chaperone system. It is encoded by the grpE gene. GrpE has an approximate molecular mass of 21 kDa. This protein is mainly expressed in the cytoplasm of bacterial cells where it plays a significant role in maintaining protein homeostasis under stress conditions such as heat shock. GrpE acts synergistically with DnaK another essential component of the bacterial chaperone machinery.
Biological function summary

GrpE acts as a nucleotide exchange factor within the chaperone system specifically with DnaK and DnaJ proteins. GrpE facilitates the release of ADP from DnaK allowing ATP binding and subsequent release of the substrate protein. This process is central to the correct folding and repair of proteins that may become misfolded or denatured. GrpE DnaK and DnaJ form a chaperone complex that is essential for protein quality control and cellular functions.

Pathways

GrpE serves a significant role in bacterial heat shock response pathways and proteostasis. It is integrally associated with the Hsp70/DnaK protein folding pathway which is vital in dealing with cellular stress and maintaining protein integrity. GrpE interacts directly with DnaK and indirectly through the DnaJ co-chaperone forming an important triad for bacterial survival under various stress conditions such as elevated temperatures.

GrpE and its associated chaperone complex have relevance in pathogenic bacteria contributing to their virulence and infection capability. For example GrpE in Escherichia coli is implicated in increased virulence supporting bacterial resistance to environmental stressors. This protein complex might interact with various bacterial virulence factors and stress proteins that could amplify the pathogen's ability to survive hostile environments such as within a host organism's immune response.
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일반 정보

기능

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.

서열 유사성

Belongs to the GrpE family.

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제품 프로토콜

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타겟 정보

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.
See full target information grpE

대체 명칭 보기

b2614, JW2594, grpE, Protein GrpE, HSP-70 cofactor, HSP24, Heat shock protein B25.3, HSP24, HSP 70 cofactor, Heat shock protein B25.3
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular cell 70:614-627.e7 PubMed29754824

2018

CnoX Is a Chaperedoxin: A Holdase that Protects Its Substrates from Irreversible Oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Camille V Goemans,Didier Vertommen,Rym Agrebi,Jean-François Collet
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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