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AB230255

Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag)

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Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) is a Full Length protein, in the 263 to 670 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for ELISA, WB, SDS-PAGE.
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ELISA - Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (AB230255)
  • ELISA

Unknown

ELISA - Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (AB230255)

Plate was coated with ab230255 starting at 800 ng/well, serially diluted in DPBS. Washed plate was detected using Rabbit Anti-EEEV E2 pAb at 1 μg/mL. OD650 is reported.

Western blot - Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (AB230255)
  • WB

Supplier Data

Western blot - Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (AB230255)

Followed by TMB substrate.

All lanes:

anti-EEEV E2 rabbit polyclonal antibody at 0.5 µg/mL

Lane 1:

Western blot - Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255) at 0.05 µg

Lane 2:

Western blot - Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255) at 0.1 µg

Lane 3:

Western blot - Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255) at 0.2 µg

Secondary

All lanes:

anti-rabbit IgG HRP conjugate

false

SDS-PAGE - Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (AB230255)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (AB230255)

SDS-PAGE analysis of 1 μg and 5 μg (lanes 1-2) of ab230255 under denaturing and reducing conditions.

Two prominent bands : E3E2 at ~50 kDa and cleaved E2 at ~40 kDa.

주요 정보

Purity

>80% SDS-PAGE

Purified by FPLC.

발현 시스템

Baculovirus infected Sf9 cells

Tags

His tag C-Terminus

Applications

ELISA, SDS-PAGE, WB

applications

Biologically active

No

Animal free

No

Carrier free

No

보관 버퍼

Constituents: PBS

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325996

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 12 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 12 bands) (AB325996)

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SDS-PAGE - Recombinant Rubella Virus capsid protein (AB74574)

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Proteins & Peptides

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Recombinant EN-TEV Protease protein

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AB256431

Recombinant Severe Fever with Thrombocytopenia Syndrome Virus Nucleoprotein (His tag)

SDS-PAGE - Recombinant Severe Fever with Thrombocytopenia Syndrome Virus Nucleoprotein (His tag) (AB256431)

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AB49036

Recombinant Hepatitis E Virus ORF2 antigen protein (Tag Free)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>E3E2 at ~50 kDa and E2 at ~40 kDa.</p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>E3E2 at ~50 kDa and E2 at ~40 kDa.</p>" } } }
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"46.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":670,"aminoAcidStart":263,"nature":"Recombinant","expressionSystem":null,"accessionNumber":null,"tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Eastern Equine Encephalitis Virus (EEEV) E3E2 is a structural protein important for the viral life cycle. E3E2 refers to the heterodimer E3 and E2 proteins. E2 is the larger component with a molecular mass of about 50 kDa. Expressed on the surface of the virus E2 facilitates host cell attachment and entry making it an important player in the infection process. The interaction of E2 with host cell receptors determines the host range of the virus. While E3 assists in the proper folding and processing of E2 it is less understood than E2.
Biological function summary

The E3E2 complex assists the virus in evading host immune responses. The complex is part of the viral envelope that shields the viral genome from host recognition. During viral replication E3E2 facilitates the fusion of the viral membrane with host cell membranes enabling viral entry. This complex is indispensable for the survival and propagation of EEEV within host cells. Although E3 does not get incorporated fully into complete virions it plays a management role in processing E2 emphasizing its importance for viral maturation.

Pathways

E3E2 participates in the viral entry and replication pathways. It functions by interacting with the host cell's surface proteins initiating membrane fusion and subsequent events leading to viral replication. E3E2 interacts with protein markers in the endocytic pathway—critical to viral internalization and uncoating. During EEEV infection E3E2 might also associate indirectly with cellular proteins such as integrins though specific partner proteins in the host pathway remain largely undefined.

E3E2 plays a significant role in the pathogenesis of Eastern Equine Encephalitis (EEE). EEEV causes severe encephalitic disease in both humans and horses with E2 being a primary target for neutralizing antibodies. The virus induces neuroinflammation leading to severe neurological symptoms. Additionally E2 variations can affect virulence and tissue tropism highlighting its role in disease severity. The EEEV infection mechanism involves host proteins such as heparin sulfate which assists E3E2 in mediating cell attachment linking it to the observed clinical manifestations of EEE.
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일반 정보

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제품 프로토콜

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타겟 정보

See full target information Eastern Equine Encephalitis Virus E3E2
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Product promise

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