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AB285976

Recombinant EN-TEV Protease protein

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Recombinant EN-TEV Protease protein is a Tobacco etch virus Fragment protein, in the 2038 to 2279 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Size Exclusion Chromatography, FuncS.
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Functional Studies - Recombinant EN-TEV Protease protein (AB285976)
  • FuncS

Supplier Data

Functional Studies - Recombinant EN-TEV Protease protein (AB285976)

TEV activity graph using ab285976

SDS-PAGE - Recombinant EN-TEV Protease protein (AB285976)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant EN-TEV Protease protein (AB285976)

4–12% SDS-PAGE : 1, 2 and 3 ug of TEV Protease loaded in each lane under reducing conditions and stained with Coomassie Blue. TEV Protease has a predicted MW of ~31 kDa

주요 정보

Purity

>95% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE, Size Exclusion Chromatography, FuncS

applications

Biologically active

Yes

Biological activity

The EZCut™ TEV Protease has an activity of ≥10 mU/mg

Accession

P04517-1

Animal free

No

Carrier free

No

Species

Tobacco etch virus

보관 버퍼

Constituents: 50% Glycerol (glycerin, glycerine), 0.037% Sodium chloride, 0.009% disodium;hydrogen phosphate;dodecahydrate, 0.001% Potassium chloride, 0.001% Potassium phosphate monobasic

storage-buffer

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Size Exclusion Chromatography": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

서열 정보

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":"31 kDa","aminoAcidEnd":2279,"aminoAcidStart":2038,"nature":"Recombinant","expressionSystem":null,"accessionNumber":null,"tags":[{"tag":"His","terminus":"C-Terminus"}]}]

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification His Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
True

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EN-TEV Protease also known simply as TEV protease is a specific proteolytic enzyme commonly utilized for its high specificity in cleaving peptide sequences. Its other popular name is Tobacco Etch Virus NIa protease and it has a molecular mass of approximately 27 kDa. TEV protease is expressed mainly in bacteria such as Escherichia coli when used in laboratory settings for protein preparation. Its ability to cleave at a specific sequence makes it invaluable in protein purification processes.
Biological function summary

The action of TEV protease involves recognizing and cleaving a specific sequence in fusion proteins allowing for the removal of affinity tags. Though not generally part of a complex its activity is often important for modifying proteins without interfering with their structure or function beyond the cleaved site. By providing a method to separate desired parts of proteins TEV protease enhances the study of protein functions and structures. It also assists in generating authentic recombinant proteins critical for various research applications.

Pathways

TEV protease's role mainly revolves around its function in post-translational modification processes rather than classical signaling pathways. It facilitates pathways where the removal of fusion tags is necessary for studying protein interactions and conformations. This function does not directly involve TEV protease in cellular metabolic pathways but its activity complements the activity of other proteases like Actev protease and protease assay protocol tools by providing a precise cleavage mechanism necessary for subsequent biochemical analysis and studies.

Although TEV protease does not directly relate to specific diseases it has a significant role in research involving proteins associated with various conditions. For instance in neurodegenerative studies researchers utilize TEV protease to isolate and study proteins potentially connected to disorders such as Alzheimer's disease. The protease substrate generated by its specific cleavage can be studied alongside proteins like tau which are linked to disease pathology. The ability to manipulate protein structures cleanly and efficiently with TEV protease underpins its utility in both clinical research and therapeutic development.

제품 프로토콜

타겟 정보

Helper component proteinase. Required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus (PubMed : 2656254). Interacts with virions and aphid stylets (PubMed : 9880030). Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs (PubMed : 11414807). May have RNA-binding activity.. Cytoplasmic inclusion protein. Has helicase activity. It may be involved in replication.. 6 kDa protein 1. Indispensable for virus replication (By similarity). Reduces the abundance of host transcripts related to jasmonic acid biosynthesis therefore altering the host defenses (By similarity). In order to increase its own stability, decreases host protein degradation pathways (By similarity).. 6 kDa protein 2. Indispensable for virus replication.. Viral genome-linked protein. Mediates the cap-independent, EIF4E-dependent translation of viral genomic RNAs (Probable). Binds to the cap-binding site of host EIF4E and thus interferes with the host EIF4E-dependent mRNA export and translation (By similarity). VPg-RNA directly binds EIF4E and is a template for transcription (By similarity). Also forms trimeric complexes with EIF4E-EIF4G, which are templates for translation (By similarity).. Nuclear inclusion protein A. Has RNA-binding and proteolytic activities.. Nuclear inclusion protein B. An RNA-dependent RNA polymerase that plays an essential role in the virus replication.. Capsid protein. Involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification.
See full target information Genome polyprotein

대체 명칭 보기

Genome polyprotein

Product promise

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