JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB238365

Recombinant Endo-beta-1,6-galactanase protein (His tag)

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Recombinant Endo-beta-1,6-galactanase protein (His tag) is a Trichoderma viride Full Length protein, in the 21 to 479 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

대체 명칭 보기

6GAL

1 이미지
SDS-PAGE - Recombinant Endo-beta-1,6-galactanase protein (His tag) (AB238365)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Endo-beta-1,6-galactanase protein (His tag) (AB238365)

ab238365 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

주요 정보

Purity

>85% SDS-PAGE

발현 시스템

Escherichia coli

Tags

10x His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Trichoderma viride

보관 버퍼

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

서열 정보

[{"linker":null,"sequence":"DTTLTIDPTSNWGTWEGWGVSLAWWAKAFGNRDDLASVFFSRNNQAVNGQTLPGLGFNIVRYNAGACSNNSYDGSTMVVSPNIKPSRQMDGFWLDWASSDPSSSSWNWNVDANQRAMLQKAKANGANIFELFSNSPMWWMCNNHNPSGSGSSDNLQSWNYQNHAVYLADIAQHAQQSWRIQFQSVEAFNEPSSSWWTAEGTQEGCHFDVSTMATVIGYLNTELSSRGLSSFVASSDENTYDLAISTWQGFNSSTRNIVKRINVHGYQDGGGRRDTLYSLASQAGKRLWNSEYGDSDASGKSMYQNLLLDFTWLHPTAWVYWQAIDGAGWGLIVGDNDNLTLSSASTKYFVLAQLTRHIRQGMQILTTPDVNTAVAYDAGSQKLVIVTANWGSAQTITFDLTRARTAGSNGATVPRWSTQTGGGDQYRSYTDTKINNGKFSASFSSGQVQTFEVSGVVLQ","proteinLength":"Full Length","predictedMolecularWeight":"56.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":479,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q76FP5","tags":[{"tag":"10x His","terminus":"N-Terminus"}]}]

특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
False

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Endo-beta-16-galactanase also known as E-B-16-gal is an enzyme that performs hydrolysis of the beta-16-galactan linkages in complex carbohydrates. The enzyme carries a mass of about 40 kDa depending on the organism of origin. This protein exhibits broad expression in various plant tissues as it plays a fundamental role in the breakdown of plant cell wall polymers. It facilitates the conversion of complex polysaccharides into simpler sugars.
Biological function summary

This enzyme functions to degrade rhamnogalacturonan-I a component of pectin within the plant cell wall matrix. Endo-beta-16-galactanase operates as part of the larger pectinase complex which includes other enzymes like polygalacturonase and pectin lyase. Through collaboration within this complex the enzyme contributes significantly to cell wall modification and redevelopment during processes such as growth fruit ripening and plant senescence.

Pathways

Endo-beta-16-galactanase plays a role in the plant cell wall degradation pathway actively participating in remodeling plant structure and facilitating plant growth. It works alongside enzymes like endo-14-beta-glucanase which also modulate the structure of the cell wall. Additionally the enzyme plays a part in the carbohydrate biosynthesis pathway ensuring the cycling of sugars needed for energy and growth.

The activity of endo-beta-16-galactanase can be linked to plant defense mechanisms and responses to pathogen attacks. It influences the integrity of the cell wall with its dysregulation potentially leading to issues with plant pathogenicity. The enzyme connects with proteins like chitinase as both participate in fortifying the plant’s defenses against fungal pathogens. Although it is extensively studied in plant systems its activity and function might have parallels in other biological contexts where it's involved in complex carbohydrate metabolism.

일반 정보

기능

Hydrolyzes galactooligomers with a degree of polymerization higher than 3. Hydrolyzes radish root arabinogalactan-protein. Does not hydrolyze dextran, arabinan, starch, laminarin, beta-1,4- and beta-1,3-galactans, larch wood arabinogalactan or acid-insoluble polygalacturonic acid.

서열 유사성

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

제품 프로토콜

타겟 정보

Hydrolyzes galactooligomers with a degree of polymerization higher than 3. Hydrolyzes radish root arabinogalactan-protein. Does not hydrolyze dextran, arabinan, starch, laminarin, beta-1,4- and beta-1,3-galactans, larch wood arabinogalactan or acid-insoluble polygalacturonic acid.
See full target information 6GAL

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com