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AB78710

Recombinant Human 14-3-3 eta/YWHAH protein (His tag N-Terminus)

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Recombinant Human 14-3-3 eta/YWHAH protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.
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SDS-PAGE - Recombinant Human 14-3-3 eta/YWHAH protein (His tag N-Terminus) (AB78710)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human 14-3-3 eta/YWHAH protein (His tag N-Terminus) (AB78710)

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

주요 정보

Purity

>95% SDS-PAGE

ab78710 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q04917-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.3152% Tris HCl

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

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  • 0
Primary Antibodies

AB32560

Anti-14-3-3 alpha + beta antibody [Y62]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 alpha + beta antibody [Y62] (AB32560)

  • 0
  • 0
Primary Antibodies

AB30554

Anti-14-3-3 (phospho S58) antibody

Immunohistochemistry (Frozen sections) - Anti-14-3-3 (phospho S58) antibody (AB30554)

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  • 1
Primary Antibodies

AB206292

Anti-14-3-3 eta/YWHAH antibody [EPR16750]

RabMAb|Recombinant

Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (AB206292)

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  • 2
Proteins & Peptides

AB163206

Recombinant Human Transmembrane protein 30A (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Transmembrane protein 30A (GST tag N-Terminus) (AB163206)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

This product was previously labelled as 14-3-3 eta
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGDREQLLQRARLAEQAERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVISSIEQKTMADGNEKKLEKVKAYREKIEKELETVCNDVLSLLDKFLIKNCNDFQYESKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISKEQMQPTHPIRLGLALNFSVFYYEIQNAPEQACLLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDEEAGEGN","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q04917","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 eta protein also known by its gene name YWHAH functions mechanically as an adapter protein involved in many cellular processes. This protein weighs approximately 28 kDa and facilitates the modulation of signaling pathways by binding to phosphoserine-containing proteins. It exhibits widespread expression with significant presence in the brain emphasizing its role in nervous system activities. The 14-3-3 eta protein acts as a hub in various signal transduction pathways influencing cell cycle regulation and apoptosis.
Biological function summary

The 14-3-3 eta protein interacts with diverse intracellular proteins forming various complexes that influence cellular functions. It plays a pivotal role in cellular stress responses and the regulation of several metabolic processes. The protein modulates activity by altering the localization of target proteins or masking their phosphorylation sites which affects their function and stability. This regulatory capacity emphasizes the protein's impact on cell survival and metabolism.

Pathways

The 14-3-3 eta protein integrates into key signaling networks such as the MAPK/ERK and PI3K/AKT pathways. These pathways are essential in mediating processes like cell proliferation differentiation and survival. Within these pathways 14-3-3 eta functions alongside proteins like RAF1 and AKT1 coordinating cellular responses to external stimuli. Its involvement in these pathways highlights the adaptability and versatile roles it plays in cellular signaling and homeostasis.

The 14-3-3 eta protein has associations with conditions such as rheumatoid arthritis and neurodegenerative diseases. In rheumatoid arthritis altered expression and interactions with other proteins like cytokines contribute to inflammatory processes. In neurodegenerative disorders the dysregulation of 14-3-3 eta affects proteins related to tau pathology influencing disease progression. Understanding these connections helps in comprehending the protein's role in disease mechanisms and developing therapeutic strategies.
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일반 정보

기능

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

서열 유사성

Belongs to the 14-3-3 family.

Post-translational modifications

Phosphorylated on Ser-59 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.

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제품 프로토콜

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타겟 정보

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
See full target information YWHAH

대체 명칭 보기

YWHA1, YWHAH, 14-3-3 protein eta, Protein AS1
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Product promise

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