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AB314608

Recombinant Human ABCD3 Protein (His Tag)

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Recombinant Human ABCD3 Protein (His Tag) is a Human Full Length protein, in the 1 to 659 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

대체 명칭 보기

PMP70, PXMP1, ABCD3, ATP-binding cassette sub-family D member 3, 70 kDa peroxisomal membrane protein

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SDS-PAGE - Recombinant Human ABCD3 Protein (His Tag) (AB314608)
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Supplier Data

SDS-PAGE - Recombinant Human ABCD3 Protein (His Tag) (AB314608)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

주요 정보

Purity

>85% SDS-PAGE

발현 시스템

Escherichia coli

Tags

10x His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

보관 버퍼

pH: 7.4 - 8 Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% 2-Octadecoxyethanol

storage-buffer

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보완 제품

Primary Antibodies

AB3421

Anti-PMP70 antibody

Western blot - Anti-PMP70 antibody (AB3421)

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  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MAAFSKYLTARNSSLAGAAFLLLCLLHKRRRALGLHGKKSGKPPLQNNEKEGKKERAVVDKVFFSRLIQILKIMVPRTFCKETGYLVLIAVMLVSRTYCDVWMIQNGTLIESGIIGRSRKDFKRYLLNFIAAMPLISLVNNFLKYGLNELKLCFRVRLTKYLYEEYLQAFTYYKMGNLDNRIANPDQLLTQDVEKFCNSVVDLYSNLSKPFLDIVLYIFKLTSAIGAQGPASMMAYLVVSGLFLTRLRRPIGKMTITEQKYEGEYRYVNSRLITNSEEIAFYNGNKREKQTVHSVFRKLVEHLHNFILFRFSMGFIDSIIAKYLATVVGYLVVSRPFLDLSHPRHLKSTHSELLEDYYQSGRMLLRMSQALGRIVLAGREMTRLAGFTARITELMQVLKDLNHGKYERTMVSQQEKGIEGVQVIPLIPGAGEIIIADNIIKFDHVPLATPNGDVLIRDLNFEVRSGANVLICGPNGCGKSSLFRVLGELWPLFGGRLTKPERGKLFYVPQRPYMTLGTLRDQVIYPDGREDQKRKGISDLVLKEYLDNVQLGHILEREGGWDSVQDWMDVLSGGEKQRMAMARLFYHKPQFAILDECTSAVSVDVEGYIYSHCRKVGITLFTVSHRKSLWKHHEYYLHMDGRGNYEFKQITEDTVEFGS","proteinLength":"Full Length","predictedMolecularWeight":"81.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":659,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P28288","tags":[{"tag":"10x His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Lyophilized
Purification 테크닉
Ion exchange chromatography
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-80°C
분주 정보
Add glycerol to a final volume of 50% for extra stability and aliquot
보관 정보
The product can be stored for up to 12 months
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PMP70 also known as ABCD3 is a peroxisomal membrane protein with a molecular weight of approximately 70 kDa. It belongs to the ATP-binding cassette (ABC) transporter family and localizes on the peroxisomal membrane predominantly expressed in liver kidney and testes. PMP70 plays an important mechanical role in the transportation of very long-chain fatty acids (VLCFAs) across the peroxisomal membrane.
Biological function summary

This protein operates as part of a heterodimeric complex with its related transporter protein ALDP (ABCD1). PMP70 facilitates the import of VLCFAs into peroxisomes which are then subjected to β-oxidation. By doing this PMP70 contributes significantly to the metabolism of lipids maintaining internal cellular homeostasis.

Pathways

PMP70 integrates into the peroxisomal β-oxidation pathway alongside other proteins such as ALDP and ACSVL1. It ensures the proper flux of VLCFAs into peroxisomes which is essential for their breakdown and energy production. The protein also interacts with metabolic pathways linked to lipid biosynthesis and degradation illustrating its multifaceted role in cellular lipid management.

PMP70's dysfunction relates to X-linked adrenoleukodystrophy (X-ALD) a severe genetic disorder affecting the nervous system and adrenal glands. Mutations in PMP70 and related proteins particularly ALDP disrupt VLCFA degradation leading to toxic accumulation. This highlights the clinical significance of PMP70 and its potential as a therapeutic target in metabolic and neurodegenerative diseases.
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일반 정보

기능

Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that catalyzes the transport of long-chain fatty acids (LCFA)-CoA, dicarboxylic acids-CoA, long-branched-chain fatty acids-CoA and bile acids from the cytosol to the peroxisome lumen for beta-oxydation (PubMed : 11248239, PubMed : 24333844, PubMed : 25168382, PubMed : 29397936). Has fatty acyl-CoA thioesterase and ATPase activities (PubMed : 29397936). Probably hydrolyzes fatty acyl-CoAs into free fatty acids prior to their ATP-dependent transport into peroxisomes (By similarity). Thus, play a role in regulation of LCFAs and energy metabolism namely, in the degradation and biosynthesis of fatty acids by beta-oxidation (PubMed : 24333844, PubMed : 25944712).

서열 유사성

Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.

Post-translational modifications

Ubiquitinated by PEX2 during pexophagy in response to starvation, leading to its degradation.

Subcellular localisation

Peroxisome membrane

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제품 프로토콜

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타겟 정보

Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that catalyzes the transport of long-chain fatty acids (LCFA)-CoA, dicarboxylic acids-CoA, long-branched-chain fatty acids-CoA and bile acids from the cytosol to the peroxisome lumen for beta-oxydation (PubMed : 11248239, PubMed : 24333844, PubMed : 25168382, PubMed : 29397936). Has fatty acyl-CoA thioesterase and ATPase activities (PubMed : 29397936). Probably hydrolyzes fatty acyl-CoAs into free fatty acids prior to their ATP-dependent transport into peroxisomes (By similarity). Thus, play a role in regulation of LCFAs and energy metabolism namely, in the degradation and biosynthesis of fatty acids by beta-oxidation (PubMed : 24333844, PubMed : 25944712).
See full target information ABCD3
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Product promise

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