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AB322756

Recombinant Human ABO Protein Standard (His tag)

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Recombinant Human ABO Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.
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Sandwich ELISA - Recombinant Human ABO Protein Standard (His tag) (AB322756)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human ABO Protein Standard (His tag) (AB322756)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human ABO Protein Standard (His tag) (AB322756)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ABO Protein Standard (His tag) (AB322756)

SDS-PAGE analysis of ab322756 under reducing conditions for 2ug protein.

주요 정보

Purity

>80% SDS-PAGE

발현 시스템

HEK 293 cells

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

P16442-1

Animal free

Yes

Carrier free

No

Species

Human

보관 버퍼

pH: 7.3 - 7.5 Constituents: 12.6% Glycerol (glycerin, glycerine), 2.922% Sodium chloride, 1.742% L-Arginine, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

  • 5
  • 1
Assay Kits

AB270207

Human ABO ELISA Kit (Histo-Blood Group ABO System Transferase)

Recombinant|SimpleStep

Sandwich ELISA - Human ABO ELISA Kit (Histo-Blood Group ABO System Transferase) (AB270207)

  • 0
  • 0
Primary Antibodies

AB2521

Anti-Blood Group A Antigen antibody [HE-193]

  • 0
  • 0
Assay Kits

AB197742

Mouse IL-1 beta ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

  • 5
  • 3
Assay Kits

AB269371

Human MMP3 ELISA Kit

Recombinant|SimpleStep

ELISA - Human MMP3 ELISA Kit (AB269371)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.

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서열 정보

[{"linker":null,"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"35.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P16442","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification His Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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일반 정보

기능

This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens : A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of N-acetyl-alpha-D-galactosamine (GalNAc)) or to the B antigen (by addition of galactose (Gal)), whereas O individuals lack such activity and express the H antigen precursor unmodified.. Catalyzes the transfer of GalNAc or Gal in an alpha1,3 linkage to the terminal Gal of all five types of naturally occurring H antigens, forming the antigenic structures of the A and B blood groups.

서열 유사성

Belongs to the glycosyltransferase 6 family.

Post-translational modifications

The soluble form derives from the membrane form by proteolytic processing.

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제품 프로토콜

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타겟 정보

This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens : A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of N-acetyl-alpha-D-galactosamine (GalNAc)) or to the B antigen (by addition of galactose (Gal)), whereas O individuals lack such activity and express the H antigen precursor unmodified.. Catalyzes the transfer of GalNAc or Gal in an alpha1,3 linkage to the terminal Gal of all five types of naturally occurring H antigens, forming the antigenic structures of the A and B blood groups.
See full target information Histo-blood group ABO system transferase

대체 명칭 보기

Histo-blood group ABO system transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Histo-blood group A transferase, Histo-blood group B transferase, NAGAT, A transferase, GTA, B transferase, GTB, ABO
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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