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AB113861

Recombinant Human Apolipoprotein A V/APOA5

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Recombinant Human Apolipoprotein A V/APOA5 is a Human Full Length protein, in the 24 to 366 aa range, expressed in HEK 293 cells, with >70%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, ELISA, WB.

대체 명칭 보기

RAP3, UNQ411/PRO773, APOA5, Apolipoprotein A-V, Apo-AV, ApoA-V, Apolipoprotein A5, Regeneration-associated protein 3

1 이미지
SDS-PAGE - Recombinant Human Apolipoprotein A V/APOA5 (AB113861)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Apolipoprotein A V/APOA5 (AB113861)

12% SDS-PAGE separation of Human Apolipoprotein A V/APOA5
1.reduced and boiled sample, 5μg/lane
2.non-reduced and non-boiled sample, 5μg/lane

주요 정보

Purity

>70% SDS-PAGE

Purity determined by densitometric image analysis

Endotoxin 레벨

< 1 EU/µg

발현 시스템

HEK 293 cells

Tags

DDDDK tag C-Terminus

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in 200 µL of water

보관 버퍼

Constituents: 0.29% Sodium chloride, 0.24% Tris

storage-buffer

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보완 제품

Proteins & Peptides

AB322097

Recombinant Mouse APOA1 Protein Standard (His tag)

Sandwich ELISA - Recombinant Mouse APOA1 Protein Standard (His tag) (AB322097)

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Proteins & Peptides

AB226264

Recombinant Mouse Apolipoprotein CIII (Tagged)

SDS-PAGE - Recombinant Mouse Apolipoprotein CIII (Tagged) (AB226264)

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  • 0
Proteins & Peptides

AB280330

Recombinant human Apolipoprotein E3

Mass Spectrometry - Recombinant human Apolipoprotein E3 (AB280330)

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  • 0
Proteins & Peptides

AB114408

Recombinant Human CETP protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human CETP protein (GST tag N-Terminus) (AB114408)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

This product was previously labelled as Apolipoprotein A V
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서열 정보

[{"linker":null,"sequence":"RKGFWDYFSQTSGDKGRVEQIHQQKMAREPATLKDSLEQDLNNMNKFLEKLRPLSGSEAPRLPQDPVGMRRQLQEELEEVKARLQPYMAEAHELVGWNLEGLRQQLKPYTMDLMEQVALRVQELQEQLRVVGEDTKAQLLGGVDEAWALLQGLQSRVVHHTGRFKELFHPYAESLVSGIGRHVQELHRSVAPHAPASPARLSRCVQVLSRKLTLKAKALHARIQQNLDQLREELSRAFAGTGTEEGAGPDPQMLSEEVRQRLQAFRQDTYLQIAAFTRAIDQETEEVQQQLAPPPPGHSAFAPEFQQTDSGKVLSKLQARLDDLWEDITHSLHDQGHSHLGDPAAADYKDDDDK","proteinLength":"Full Length","predictedMolecularWeight":"40.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":366,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q6Q788","tags":[{"tag":"DDDDK","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A V also known as APOA5 plays a critical role in lipid metabolism. This protein possesses a molecular weight of approximately 39 kDa and is mainly expressed in the liver. APOA5 modulates the plasma triglyceride levels by enhancing the uptake and clearance of triglyceride-rich lipoproteins. The protein is located at the surface of these lipoproteins facilitating their interaction with receptors and enzymes like lipoprotein lipase.
Biological function summary

APOA5 influences triglyceride metabolism and is part of the HDL particle complex. It interacts directly with lipoprotein lipase to promote the hydrolysis of triglycerides into free fatty acids. This hydrolytic action aids in reducing triglyceride levels in the plasma. APOA5 also indirectly influences very-low-density lipoprotein (VLDL) metabolism reinforcing its role in lipid regulation.

Pathways

APOA5 integrates into the lipoprotein metabolic pathway and the PPAR signaling pathway. In the lipoprotein metabolic pathway APOA5 works with proteins like APOC3 to influence triglyceride homeostasis. This protein is modulated by PPAR-alpha helping control lipid metabolism and energy homeostasis. Its role in these pathways highlights its impact on triglyceride-rich lipoprotein metabolism positioning it as an important player in maintaining lipid balance.

APOA5 strongly associates with hypertriglyceridemia and cardiovascular disease. Variations in the APOA5 gene can lead to abnormal plasma triglyceride levels contributing to the development of hypertriglyceridemia. Additionally APOA5's connection with APOE proteins through lipid metabolism pathways can influence susceptibility to cardiovascular disorders. Understanding the interactions and mutations within APOA5 provides insights into the mechanisms contributing to these disease states.
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일반 정보

기능

Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and an inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity). Activates poorly lecithin : cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Binds heparin (PubMed : 17326667).

서열 유사성

Belongs to the apolipoprotein A1/A4/E family.

Post-translational modifications

Phosphorylated by FAM20C in the extracellular medium.

Subcellular localisation

Early endosome

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제품 프로토콜

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타겟 정보

Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and an inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity). Activates poorly lecithin : cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Binds heparin (PubMed : 17326667).
See full target information APOA5
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Immunity 56:2523-2541.e8 PubMed37924812

2023

Gasdermin D permeabilization of mitochondrial inner and outer membranes accelerates and enhances pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Miao,Cong Jiang,Winston Y Chang,Haiwei Zhang,Jinsu An,Felicia Ho,Pengcheng Chen,Han Zhang,Caroline Junqueira,Dulguun Amgalan,Felix G Liang,Junbing Zhang,Charles L Evavold,Iva Hafner-Bratkovič,Zhibin Zhang,Pietro Fontana,Shiyu Xia,Markus Waldeck-Weiermair,Youdong Pan,Thomas Michel,Liron Bar-Peled,Hao Wu,Jonathan C Kagan,Richard N Kitsis,Peng Zhang,Xing Liu,Judy Lieberman
제품이 사용된 논문 모두 보기
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Product promise

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