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AB116199

Recombinant Human APPBP1 protein (His tag N-Terminus)

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Recombinant Human APPBP1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 534 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

대체 명칭 보기

APPBP1, HPP1, NAE1, NEDD8-activating enzyme E1 regulatory subunit, Amyloid protein-binding protein 1, Proto-oncogene protein 1, APP-BP1

1 이미지
SDS-PAGE - Recombinant Human APPBP1 protein (His tag N-Terminus) (AB116199)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human APPBP1 protein (His tag N-Terminus) (AB116199)

15% SDS-PAGE analysis of 3 µg ab116199.

주요 정보

Purity

>90% SDS-PAGE

ab116199 was purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Primary Antibodies

AB187142

Anti-APPBP1 antibody [EPR15732]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-APPBP1 antibody [EPR15732] (AB187142)

  • 0
  • 0
Primary Antibodies

AB131161

Anti-SIM2 antibody [EPR7878]

RabMAb|Recombinant

Western blot - Anti-SIM2 antibody [EPR7878] (AB131161)

  • 0
  • 0
Primary Antibodies

AB109507

Anti-UBE2M/UBC12 antibody [EPR5333]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-UBE2M/UBC12 antibody [EPR5333] (AB109507)

  • 0
  • 0
Primary Antibodies

AB76315

Anti-STAT3 (phospho Y705) antibody [EP2147Y]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho Y705) antibody [EP2147Y] (AB76315)

  • 4
  • 21
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAQLGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL","proteinLength":"Full Length","predictedMolecularWeight":"62.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":534,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q13564","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein 'APPBP1' also known as APP-binding protein 1 functions as part of cellular processes through its role in protein modification. APPBP1 forms a dimeric complex with Uba3 together activating NEDD8 which is a small ubiquitin-like modifier. This protein has a molecular weight of approximately 58 kDa and is widely expressed across various tissues including the brain and liver. Its comprehensive roles make it essential for normal cellular operations.
Biological function summary

APPBP1 interacts within a complex known as the NEDD8-activating enzyme. This complex is essential in the neddylation pathway influencing protein degradation through the ubiquitin-proteasome system. By conjugating NEDD8 to target proteins APPBP1 controls many processes such as cell cycle regulation signal transduction and transcription. It is of special interest in research involving cellular signaling because neddylation modifies the activity and stability of many substrate proteins involved in these critical cellular functions.

Pathways

The involvement of APPBP1 in ubiquitin-like protein pathways highlights its influence on cellular homeostasis. The protein is associated with the neddylation and ubiquitination pathways influencing other members such as cullin proteins involved in cell cycle control and other cellular processes. APPBP1-related interactions play a significant role in ensuring the timely degradation of specific proteins maintaining balance within the cell's regulatory mechanisms.

Abnormalities in APPBP1 activity have been linked to neurodegenerative diseases such as Alzheimer's disease and cancer. In Alzheimer's disease APPBP1 is related to the APP protein and altered neddylation might contribute to disease pathology through dysfunctional protein degradation pathways. In cancer disrupted neddylation can result in uncontrolled cell proliferation due to improper regulation of cell cycle pathways. Understanding the function of APPBP1 could provide insights into developing therapeutic strategies for these conditions.
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일반 정보

기능

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. The covalent attachment of NEDD8 to target proteins is known as 'neddylation' and the process is involved in the regulation of cell growth, viability and development.

서열 유사성

Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.

Post-translational modifications

Ubiquitinated by TRIP12, leading to its degradation by the proteasome.

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제품 프로토콜

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타겟 정보

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. The covalent attachment of NEDD8 to target proteins is known as 'neddylation' and the process is involved in the regulation of cell growth, viability and development.
See full target information NAE1
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Product promise

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