JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB316510

Recombinant Human Arg2 Protein Standard

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Recombinant Human Arg2 Protein Standard is a Human Fragment protein, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, sELISA.

대체 명칭 보기

Arginase II, Kidney-type arginase, Non-hepatic arginase, Type II arginase, ARG2

2 이미지
Sandwich ELISA - Recombinant Human Arg2 Protein Standard (AB316510)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Arg2 Protein Standard (AB316510)

Sandwich ELISA with the capture antibody dilution at 2 μg/mL and detector antibody dilution at 0.5 μg/mL.

SDS-PAGE - Recombinant Human Arg2 Protein Standard (AB316510)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Arg2 Protein Standard (AB316510)

SDS-PAGE analysis of ab316510 under reducing conditions for 2ug protein

주요 정보

Purity

>80% SDS-PAGE

발현 시스템

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, sELISA

applications

Biologically active

No

Accession

Animal free

Yes

Carrier free

No

Species

Human

보관 버퍼

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

style
left-column

보완 제품

Reagents, Controls & Accessories

AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

  • 0
  • 0
Sample Prep & Detection Kits

AB201796

Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link®

Conjugation - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (AB201796)

  • 3
  • 1
Assay Kits

AB253756

Human Arg2 Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human Arg2 Antibody Pair - BSA and Azide free (AB253756)

  • 0
  • 0
Primary Antibodies

AB264066

Anti-Arg2 antibody [EPR22626-38]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody [EPR22626-38] (AB264066)

  • 5
  • 1
Assay Kits

AB289702

Human Arg2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Arg2 ELISA Kit (AB289702)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

style
left-column

서열 정보

[{"linker":null,"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"36.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P78540","tags":[]}]
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification His Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Arginase 2 (Arg2) also known as AII is an enzyme with a molecular weight of approximately 39 kDa. It is part of the urea cycle and functions to hydrolyze L-arginine into L-ornithine and urea. Arg2 is a mitochondrial enzyme mainly expressed in the liver kidney and prostate. Its expression can extend to other tissues such as the small intestine in some conditions and its activity is important in regulating arginine availability for various physiological processes.
Biological function summary

Arg2 plays a role in nitrogen disposal and polyamine synthesis. As part of an enzymatic complex it contributes to the urea cycle which is important for the removal of excess nitrogen from the body. Arginase activity also influences the balance of nitric oxide synthesis as it competes with nitric oxide synthase for their common substrate L-arginine. This competition affects cellular functions which rely on nitric oxide signaling for developing proper vascular and immune responses.

Pathways

Arg2 is an important player in the urea cycle and the polyamine synthesis pathway. It works alongside the enzymes involved in these processes such as ornithine decarboxylase. Additionally Arg2 has a role in the nitric oxide regulation pathway modulating nitric oxide levels by limiting the availability of L-arginine for nitric oxide synthase. This makes Arg2 essential for metabolic processes and cellular communication.

Altered Arg2 expression has been associated with cardiovascular diseases and renal complications. For instance disruptions in Arg2 function can lead to endothelial dysfunction influencing conditions like atherosclerosis. Arg2 imbalances are also linked to kidney diseases possibly due to its critical role in ammonia detoxification and renal arginine metabolism. Its interaction with other proteins such as nitric oxide synthase further emphasizes its relevance in these health conditions.
style
left-column

일반 정보

기능

May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS). Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated CD4(+) and CD8(+) T cells (PubMed : 27745970). May suppress inflammation-related signaling in asthmatic airway epithelium (PubMed : 27214549). May contribute to the immune evasion of H.pylori by restricting M1 macrophage activation and polyamine metabolism (By similarity). In fetal dendritic cells may play a role in promoting immune suppression and T cell TNF production during gestation (PubMed : 28614294). Regulates RPS6KB1 signaling, which promotes endothelial cell senescence and inflammation and implicates NOS3/eNOS dysfunction (PubMed : 22928666). Can inhibit endothelial autophagy independently of its enzymatic activity implicating mTORC2 signaling (PubMed : 25484082). Involved in vascular smooth muscle cell senescence and apoptosis independently of its enzymatic activity (PubMed : 23832324). Since NOS is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase-2 plays a role in both male and female sexual arousal (PubMed : 12859189).

서열 유사성

Belongs to the arginase family.

Subcellular localisation

Mitochondrion

style
left-column

제품 프로토콜

style
left-column

타겟 정보

May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS). Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated CD4(+) and CD8(+) T cells (PubMed : 27745970). May suppress inflammation-related signaling in asthmatic airway epithelium (PubMed : 27214549). May contribute to the immune evasion of H.pylori by restricting M1 macrophage activation and polyamine metabolism (By similarity). In fetal dendritic cells may play a role in promoting immune suppression and T cell TNF production during gestation (PubMed : 28614294). Regulates RPS6KB1 signaling, which promotes endothelial cell senescence and inflammation and implicates NOS3/eNOS dysfunction (PubMed : 22928666). Can inhibit endothelial autophagy independently of its enzymatic activity implicating mTORC2 signaling (PubMed : 25484082). Involved in vascular smooth muscle cell senescence and apoptosis independently of its enzymatic activity (PubMed : 23832324). Since NOS is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase-2 plays a role in both male and female sexual arousal (PubMed : 12859189).
See full target information ARG2
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com