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AB225977

Recombinant Human ATG14L protein (Tagged)

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Recombinant Human ATG14L protein (Tagged) is a Human Full Length protein, in the 1 to 492 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

대체 명칭 보기

ATG14L, KIAA0831, ATG14, Beclin 1-associated autophagy-related key regulator, Barkor, Autophagy-related protein 14-like protein, Atg14L

1 이미지
SDS-PAGE - Recombinant Human ATG14L protein (Tagged) (AB225977)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ATG14L protein (Tagged) (AB225977)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225977 with 5% enrichment gel and 15% separation gel.

주요 정보

Purity

>90% SDS-PAGE

발현 시스템

Escherichia coli

Tags

10x His tag N-Terminus Myc tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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보완 제품

Cell Lines & Lysates

AB258319

Human ATG14 (ATG14L) knockout HeLa cell lysate

Western blot - Human ATG14 (ATG14L) knockout HeLa cell lysate (AB258319)

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Primary Antibodies

AB315009

Anti-ATG14 antibody [EPR26188-66]

20ul selling size|RabMAb|KO Validated|Recombinant

Flow Cytometry (Intracellular) - Anti-ATG14 antibody [EPR26188-66] (AB315009)

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Primary Antibodies

AB273023

Anti-IL-2RG antibody [EPR24694-133]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2RG antibody [EPR24694-133] (AB273023)

  • 0
  • 0
Primary Antibodies

AB203831

Anti-KCNIP4 antibody [EPR18314]

RabMAb|Recombinant

Immunoprecipitation - Anti-KCNIP4 antibody [EPR18314] (AB203831)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MASPSGKGARALEAPGCGPRPLARDLVDSVDDAEGLYVAVERCPLCNTTRRRLTCAKCVQSGDFVYFDGRDRERFIDKKERLSRLKSKQEEFQKEVLKAMEGKWITDQLRWKIMSCKMRIEQLKQTICKGNEEMEKNSEGLLKTKEKNQKLYSRAQRHQEKKEKIQRHNRKLGDLVEKKTIDLRSHYERLANLRRSHILELTSVIFPIEEVKTGVRDPADVSSESDSAMTSSTVSKLAEARRTTYLSGRWVCDDHNGDTSISITGPWISLPNNGDYSAYYSWVEEKKTTQGPDMEQSNPAYTISAALCYATQLVNILSHILDVNLPKKLCNSEFCGENLSKQKFTRAVKKLNANILYLCFSQHVNLDQLQPLHTLRNLMYLVSPSSEHLGRSGPFEVRADLEESMEFVDPGVAGESDESGDERVSDEETDLGTDWENLPSPRFCDIPSQSVEVSQSQSTQASPPIASSSAGGMISSAAASVTSWFKAYTGHR","proteinLength":"Full Length","predictedMolecularWeight":"60.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":492,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q6ZNE5","tags":[{"tag":"10x His","terminus":"N-Terminus"},{"tag":"Myc","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ATG14L also known as BARKOR is an essential protein involved in the formation of autophagosomes organelles which sequester cytoplasmic components for degradation. This protein is approximately 55 kDa in mass. ATG14L is expressed in a variety of tissues including high expression in the brain heart and kidney. It plays an important role in mediating the recruitment of the PI3K (phosphatidylinositol 3-kinase) complex to the isolation membrane a step necessary in autophagy initiation.
Biological function summary

ATG14L coordinates with other proteins as part of the PI3K complex performing a central function in the regulation of autophagy the cellular degradation process critical for maintaining cellular homeostasis. This complex includes VPS34 VPS15 and Beclin 1 which work together to facilitate the nucleation of the autophagosome membrane. Autophagy driven by ATG14L is important for cellular responses to nutrient deprivation and stress.

Pathways

ATG14L is important for two central pathways: the regulation of autophagy and the PI3K/AKT/mTOR signaling pathways. ATG14L directly affects the activity of mTORC1 an important regulator of cell growth and metabolism integrating signals from nutrients and growth factors. It interacts with other autophagy-related proteins such as Beclin 1 to modulate these pathways connecting intracellular energy status with metabolic processes.

ATG14L has a significant connection to neurodegenerative diseases and cancer. Altered autophagy with ATG14L malfunction contributes to the progression of neurodegenerative diseases like Alzheimer's disease where abnormal protein aggregates are not efficiently degraded. In the context of cancer dysregulation of ATG14L-related autophagy pathways can either promote or inhibit tumor growth making it an important therapeutic target. It shares complex interactions with Beclin 1 in these disorders further emphasizing its importance in disease mechanisms.
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일반 정보

기능

Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed : 18843052, PubMed : 19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed : 19270696, PubMed : 20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed : 20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed : 23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed : 23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed : 25686604, PubMed : 37632749). Modulates the hepatic lipid metabolism (By similarity).

서열 유사성

Belongs to the ATG14 family.

Post-translational modifications

Ubiquitinated via 'Lys-6', 'Lys-11' and 'Lys-63'-linked polyubiquitin chains on multiple lysines by MARCHF7, leading to ATG14 aggregation and loss of interaction with STX17.

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제품 프로토콜

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타겟 정보

Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed : 18843052, PubMed : 19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed : 19270696, PubMed : 20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed : 20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed : 23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed : 23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed : 25686604, PubMed : 37632749). Modulates the hepatic lipid metabolism (By similarity).
See full target information ATG14
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Product promise

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