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AB196096

Recombinant human BRD3 protein (His tag N-Terminus)

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Recombinant human BRD3 protein (His tag N-Terminus) is a Human Fragment protein, in the 29 to 417 aa range, expressed in Escherichia coli, with >37%, suitable for SDS-PAGE, FuncS.

대체 명칭 보기

KIAA0043, RING3L, BRD3, Bromodomain-containing protein 3, RING3-like protein

2 이미지
Functional Studies - Recombinant human BRD3 protein (His tag N-Terminus) (AB196096)
  • FuncS

Supplier Data

Functional Studies - Recombinant human BRD3 protein (His tag N-Terminus) (AB196096)

BRD3 protein is incubated with biotinylated acetyl-histone peptide substrate in BRD assay buffer in a 10 μl reaction for 1 hour at RT. Ni acceptor beads are added, followed by Streptavidin-conjugated donor beads. After 1 hour incubation at RT, Alphacounts are measured.

SDS-PAGE - Recombinant human BRD3 protein (His tag N-Terminus) (AB196096)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human BRD3 protein (His tag N-Terminus) (AB196096)

SDS PAGE analysis of ab196096.

주요 정보

Purity

>37% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Active

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.2 Constituents: 20% Glycerol (glycerin, glycerine), 1.68% Sodium chloride, 0.95% HEPES

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

  • 0
  • 0
Proteins & Peptides

AB176936

Recombinant Human Brd4 protein

SDS-PAGE - Recombinant Human Brd4 protein (AB176936)

  • 0
  • 0
Proteins & Peptides

AB196109

Recombinant human BRD2 protein (His tag N-Terminus)

Functional Studies - Recombinant human BRD2 protein (His tag N-Terminus) (AB196109)

  • 0
  • 0
Proteins & Peptides

AB243114

Recombinant human beta Defensin 1 protein (Active)

Bioactive

SDS-PAGE - Recombinant human beta Defensin 1 protein (Active) (AB243114)

  • 0
  • 0
Proteins & Peptides

AB255991

Recombinant Human BD-3 protein

SDS-PAGE - Recombinant Human BD-3 protein (AB255991)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.</p>" } } }
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서열 정보

[{"linker":null,"sequence":"MHHHHHHPSKPGRKTNQLQYMQNVVVKTLWKHQFAWPFYQPVDAIKLNLPDYHKIIKNPMDMGTIKKRLENNYYWSASECMQDFNTMFTNCYIYNKPTDDIVLMAQALEKIFLQKVAQMPQEEVELLPPAPKGKGRKPAAGAQSAGTQQVAAVSSVSPATPFQSVPPTVSQTPVIAATPVPTITANVTSVPVPPAAAPPPPATPIVPVVPPTPPVVKKKGVKRKADTTTPTTSAITASRSESPPPLSDPKQAKVVARRESGGRPIKPPKKDLEDGEVPQHAGKKGKLSEHLRYCDSILREMLSKKHAAYAWPFYKPVDAEALELHDYHDIIKHPMDLSTVKRKMDGREYPDAQGFAADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKM","proteinLength":"Fragment","predictedMolecularWeight":"43.95 kDa","actualMolecularWeight":null,"aminoAcidEnd":417,"aminoAcidStart":29,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q15059","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
보관 정보
Avoid freeze / thaw cycle
True
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일반 정보

기능

Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures (PubMed : 18406326, PubMed : 22464331, PubMed : 27105114, PubMed : 32895492). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed : 29567837, PubMed : 32895492). In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (PubMed : 18406326). Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT : BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression (PubMed : 32895492). Also binds non-histones acetylated proteins, such as GATA1 and GATA2 : regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity).

서열 유사성

Belongs to the BET family.

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures (PubMed : 18406326, PubMed : 22464331, PubMed : 27105114, PubMed : 32895492). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed : 29567837, PubMed : 32895492). In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (PubMed : 18406326). Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT : BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression (PubMed : 32895492). Also binds non-histones acetylated proteins, such as GATA1 and GATA2 : regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity).
See full target information BRD3
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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