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AB316560

Recombinant Human C7 Protein Standard (His tag)

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Recombinant Human C7 Protein Standard (His tag) is a Human Full Length protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.

대체 명칭 보기

Complement component C7, C7

2 이미지
Sandwich ELISA - Recombinant Human C7 Protein Standard (His tag) (AB316560)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human C7 Protein Standard (His tag) (AB316560)
SDS-PAGE - Recombinant Human C7 Protein Standard (His tag) (AB316560)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human C7 Protein Standard (His tag) (AB316560)

SDS-PAGE analysis of ab316560 under reducing conditions for 2ug protein.

주요 정보

Purity

>80% SDS-PAGE

발현 시스템

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, sELISA

applications

Biologically active

No

Accession

Animal free

Yes

Carrier free

No

Species

Human

보관 버퍼

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

  • 5
  • 1
Assay Kits

AB304938

Human C7 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human C7 ELISA Kit (AB304938)

  • 0
  • 0
Primary Antibodies

AB288721

Anti-C7 antibody [EPR25751-33] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-C7 antibody [EPR25751-33] - BSA and Azide free (Detector) (AB288721)

  • 0
  • 0
Proteins & Peptides

AB276226

Recombinant Human DC-SIGN protein (Fc Chimera)

SDS-PAGE - Recombinant Human DC-SIGN protein (Fc Chimera) (AB276226)

  • 0
  • 0
Proteins & Peptides

AB113120

Recombinant Human SNRPD3/Sm-D3 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human SNRPD3/Sm-D3 protein (His tag N-Terminus) (AB113120)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

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서열 정보

[{"linker":null,"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"92.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P10643","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification His Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Complement component 7 (C7) with a molecular mass of approximately 93 kDa plays an essential mechanical role in the complement system a critical part of innate immunity. C7 is one of the late components of the complement cascade involved in the formation of the membrane attack complex (MAC). It is primarily expressed in the liver and found in the blood plasma. By associating with other components C5b and C6 C7 anchors into the lipid bilayer of target cells serving as a foothold for further MAC assembly.
Biological function summary

C7 functions as an important facilitator in the innate immune response being an integral part of the membrane attack complex (MAC). This complex forms transmembrane channels in the cell membrane of pathogens leading to their lysis and destruction. Through its interaction with complement components like C5b and C6 C7 enables the insertion and stabilization of the MAC. This process is important for the immune system’s ability to eliminate pathogens quickly and effectively protecting the host from microbial invaders.

Pathways

The complement system pathway is the primary biological pathway involving C7. Within this pathway C7 interacts closely with complement proteins C5 and C6 contributing to the terminal pathway that leads to pathogen lysis. Besides it is linked with the classical and alternative complement pathways both of which converge at the activation of C5. Through these interactions and processes C7 works synergistically with other complement proteins to execute robust immune defense mechanisms.

C7 deficiencies have been associated with an increased susceptibility to infections particularly by Neisseria species due to impaired MAC formation. This link highlights the importance of C7 for adequate host defense. Additionally overactivation of the complement system potentially involving C7 contributes to the pathology of autoimmune disorders like systemic lupus erythematosus. In such conditions dysregulation involving proteins like C5b and C6 can lead to tissue damage and inflammation further illustrating the importance of C7 in maintaining immune homeostasis.
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일반 정보

기능

Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 22832194, PubMed : 26841837, PubMed : 27052168, PubMed : 30552328, PubMed : 3335508). The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways (PubMed : 22832194, PubMed : 30552328, PubMed : 3335508, PubMed : 39914456, PubMed : 39814882). The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 22832194, PubMed : 30552328, PubMed : 3335508). C7 serves as a membrane anchor (PubMed : 30552328). During MAC assembly, associates with C5b and C6 to form the C5b-7 complex, a key lipophilic precursor of the MAC complex, which associates with the outer leaflet and reduces the energy for membrane bending (PubMed : 30552328, PubMed : 32569291).

서열 유사성

Belongs to the complement C6/C7/C8/C9 family.

Post-translational modifications

C-, N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

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제품 프로토콜

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타겟 정보

Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 22832194, PubMed : 26841837, PubMed : 27052168, PubMed : 30552328, PubMed : 3335508). The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways (PubMed : 22832194, PubMed : 30552328, PubMed : 3335508, PubMed : 39914456, PubMed : 39814882). The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 22832194, PubMed : 30552328, PubMed : 3335508). C7 serves as a membrane anchor (PubMed : 30552328). During MAC assembly, associates with C5b and C6 to form the C5b-7 complex, a key lipophilic precursor of the MAC complex, which associates with the outer leaflet and reduces the energy for membrane bending (PubMed : 30552328, PubMed : 32569291).
See full target information C7
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Product promise

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