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AB104025

Recombinant human Casein Kinase 1 gamma 2 protein (GST tag N-Terminus)

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Recombinant human Casein Kinase 1 gamma 2 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 415 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for WB, FuncS.
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Functional Studies - Recombinant human Casein Kinase 1 gamma 2 protein (GST tag N-Terminus) (AB104025)
  • FuncS

Unknown

Functional Studies - Recombinant human Casein Kinase 1 gamma 2 protein (GST tag N-Terminus) (AB104025)

The Specific activity of ab104025 was determined to be 30 nmol/min/mg.

SDS-PAGE - Recombinant human Casein Kinase 1 gamma 2 protein (GST tag N-Terminus) (AB104025)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Casein Kinase 1 gamma 2 protein (GST tag N-Terminus) (AB104025)

SDS-PAGE showing ab104025 at approximately 73kDa.

주요 정보

Purity

>80% SDS-PAGE

Purity was determined to be >85% by densitometry. Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB, FuncS

applications

Biologically active

Yes

Biological activity

The Specific activity of ab104025 was determined to be 30 nmol/min/mg.

Accession

P78368-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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보완 제품

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AB60837

Recombinant human PKA beta (catalytic subunit) protein (GST tag N-Terminus)

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AB105125

Recombinant human CSNK2A2 protein (GST tag N-Terminus)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"73 kDa","actualMolecularWeight":null,"aminoAcidEnd":415,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P78368","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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일반 정보

기능

Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation (PubMed : 15077195, PubMed : 15917222, PubMed : 18794808, PubMed : 19005213). Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate (By similarity). Involved in regulation of reactive oxygen species (ROS) levels (PubMed : 37099597).

서열 유사성

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.

Post-translational modifications

Autophosphorylated (PubMed:18794808). Phosphorylated by aPKC which promotes dissociation from the cell cortex (PubMed:26481050).

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제품 프로토콜

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타겟 정보

Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation (PubMed : 15077195, PubMed : 15917222, PubMed : 18794808, PubMed : 19005213). Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate (By similarity). Involved in regulation of reactive oxygen species (ROS) levels (PubMed : 37099597).
See full target information CSNK1G2

대체 명칭 보기

CK1G2, CSNK1G2, Casein kinase I isoform gamma-2, CKI-gamma 2
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Product promise

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