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AB323143

Recombinant Human CD226 Protein Standard (His tag)

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Recombinant Human CD226 Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.
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Sandwich ELISA - Recombinant Human CD226 Protein Standard (His tag) (AB323143)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human CD226 Protein Standard (His tag) (AB323143)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human CD226 Protein Standard (His tag) (AB323143)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human CD226 Protein Standard (His tag) (AB323143)

SDS-PAGE analysis of ab323143 under reducing conditions for 2ug protein.

주요 정보

Purity

>80% SDS-PAGE

발현 시스템

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, sELISA

applications

Biologically active

No

Accession

Q15762-1

Animal free

Yes

Carrier free

No

Species

Human

보관 버퍼

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB234617

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB234617)

  • 0
  • 0
Proteins & Peptides

AB271455

Recombinant Human CD96 protein (Biotin) (Avi tag C-Terminus + Fc tag C-Terminus)

SDS-PAGE - Recombinant Human CD96 protein (Biotin) (Avi tag C-Terminus + Fc tag C-Terminus) (AB271455)

  • 0
  • 0
Primary Antibodies

AB212077

Anti-CD226 antibody [EPR20710]

RabMAb|Recombinant|20ul selling size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [EPR20710] (AB212077)

  • 0
  • 0
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Primary Antibodies

AB315218

Anti-CD96 antibody [EPR26489-23]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD96 antibody [EPR26489-23] (AB315218)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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서열 정보

[{"linker":null,"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"28.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q15762","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification His Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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일반 정보

기능

Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed : 8673704, PubMed : 9712030). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed : 26755705, PubMed : 31253644, PubMed : 30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity).

Post-translational modifications

PKC-mediated phosphorylation at Ser-329 is required for lipid raft recruitment (PubMed:15684041). Phosphorylation of Tyr-322 requires association with lipid rafts and allows GRB2 binding and activation of subsequent signaling (By similarity).

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제품 프로토콜

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타겟 정보

Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed : 8673704, PubMed : 9712030). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed : 26755705, PubMed : 31253644, PubMed : 30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity).
See full target information CD226

대체 명칭 보기

CD226, DNAM1, CD226 antigen, DNAX accessory molecule 1, DNAM-1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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