JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB288813

Recombinant Human CD80 protein (Active)

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Recombinant Human CD80 protein (Active) is a Human Fragment protein, in the 35 to 242 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for FuncS.
3 이미지
Functional Studies - Recombinant Human CD80 protein (Active) (AB288813)
  • FuncS

Supplier Data

Functional Studies - Recombinant Human CD80 protein (Active) (AB288813)

Loaded Human CD28, Human Fc Tag on Protein A Biosensor can bind Human CD80 with an affinity constant of 22.5 uM as determined in BLI assay (GatorBio Prime).

Mass Spectrometry - Recombinant Human CD80 protein (Active) (AB288813)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human CD80 protein (Active) (AB288813)

Mass determination by ESI-TOF.

Predicted MW is 23922.11 Da. (+/- 10 Da by ESI-TOF). Observed MW is 23928.41 Da.

SDS-PAGE - Recombinant Human CD80 protein (Active) (AB288813)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human CD80 protein (Active) (AB288813)

SDS-PAGE analysis of ab288813

주요 정보

Purity

>95% SDS-PAGE

Endotoxin 레벨

<0.005 EU/µg

발현 시스템

HEK 293 cells

Tags

Tag free

Applications

FuncS

applications

Biologically active

Yes

Biological activity

Loaded Human CD28, Human Fc Tag on Protein A Biosensor can bind Human CD80 with an affinity constant of 22.5 uM as determined in BLI assay (GatorBio Prime).

Accession

P33681-1

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

보관 버퍼

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

style
left-column

보완 제품

Primary Antibodies

AB288358

Alexa Fluor® 647 Anti-CD86 antibody [EPR21962]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD86 antibody [EPR21962] (AB288358)

  • 0
  • 0
Proteins & Peptides

AB307475

Recombinant Human CD86 protein (Fc Chimera)

Mass Spectrometry - Recombinant Human CD86 protein (Fc Chimera) (AB307475)

  • 0
  • 0
Proteins & Peptides

AB315080

Recombinant Mouse CD40L/TRAP Protein

Mass Spectrometry - Recombinant Mouse CD40L/TRAP Protein (AB315080)

  • 0
  • 0
Primary Antibodies

AB270112

Alexa Fluor® 488 Anti-CD80 antibody [EPR22183]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD80 antibody [EPR22183] (AB270112)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

서열 정보

[{"linker":null,"sequence":"VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDN","proteinLength":"Fragment","predictedMolecularWeight":"23.92 kDa","actualMolecularWeight":"23.93 kDa","aminoAcidEnd":242,"aminoAcidStart":35,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P33681","tags":[]}]
style
left-column

특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
Ambient
적절한 장기 보관 조건
Ambient
True
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.
Biological function summary

CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.

Pathways

CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.

CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.
style
left-column

제품 프로토콜

style
left-column

타겟 정보

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed : 38467718). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 12196291). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines (PubMed : 10438913). Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming (PubMed : 10583602, PubMed : 11279502). In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway (PubMed : 12121659). Also acts as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses (PubMed : 36727298). Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (By similarity).. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
See full target information CD80

대체 명칭 보기

CD80, CD28LG, CD28LG1, LAB7, T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, B7
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com