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AB59979

Recombinant human CDC42 binding protein kinase alpha (GST tag N-Terminus)

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Recombinant human CDC42 binding protein kinase alpha (GST tag N-Terminus) is a Human Fragment protein, in the 1 to 473 aa range, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, FuncS.
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Functional Studies - Recombinant human CDC42 binding protein kinase alpha (GST tag N-Terminus) (AB59979)
  • FuncS

Unknown

Functional Studies - Recombinant human CDC42 binding protein kinase alpha (GST tag N-Terminus) (AB59979)

The specific activity of CDC42 binding protein kinase alpha (ab59979) was determined to be 86 nmol/min/mg as per activity assay protocol

Functional Studies - Recombinant human CDC42 binding protein kinase alpha (GST tag N-Terminus) (AB59979)
  • FuncS

Supplier Data

Functional Studies - Recombinant human CDC42 binding protein kinase alpha (GST tag N-Terminus) (AB59979)

Sample Kinase Activity Plot.

SDS-PAGE - Recombinant human CDC42 binding protein kinase alpha (GST tag N-Terminus) (AB59979)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CDC42 binding protein kinase alpha (GST tag N-Terminus) (AB59979)

SDS PAGE analysis of ab59979

SDS-PAGE - Recombinant human CDC42 binding protein kinase alpha (GST tag N-Terminus) (AB59979)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CDC42 binding protein kinase alpha (GST tag N-Terminus) (AB59979)

ab59979 on SDS-PAGE.

주요 정보

Purity

>75% Densitometry

Purified using an N-terminal enzyme tag. Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity: 101nmol/min/mg.

Accession

Q5VT25-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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보완 제품

Primary Antibodies

AB96659

Anti-CDC42 binding protein kinase alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 binding protein kinase alpha antibody (AB96659)

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Primary Antibodies

AB187643

Anti-CDC42 antibody [EPR15620]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 antibody [EPR15620] (AB187643)

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Proteins & Peptides

AB85755

Recombinant human Dystrophia myotonica protein kinase / DMPK (GST tag N-Terminus)

Functional Studies - Recombinant human Dystrophia myotonica protein kinase / DMPK (GST tag N-Terminus) (AB85755)

  • 0
  • 0
Primary Antibodies

AB40795

Anti-PAK1 (phospho S144) + PAK2 (phospho S141) + PAK3 (phospho S154) antibody [EP656Y]

20ul selling size|RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-PAK1 (phospho S144) + PAK2 (phospho S141) + PAK3 (phospho S154) antibody [EP656Y] (AB40795)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Kinase assay.</p>" } } }
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제품 세부 정보

ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":473,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q5VT25","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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일반 정보

기능

Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration (PubMed : 15723050, PubMed : 9092543, PubMed : 9418861). Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2 (PubMed : 21457715). In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (PubMed : 18854160). Phosphorylates : PPP1R12A, LIMK1 and LIMK2 (PubMed : 11340065, PubMed : 11399775). May play a role in TFRC-mediated iron uptake (PubMed : 20188707). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity). Triggers the formation of an extrusion apical actin ring required for epithelial extrusion of apoptotic cells (PubMed : 29162624).

서열 유사성

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.

Post-translational modifications

Proteolytically cleaved by caspases upon apoptosis induction. The cleavage at Asp-478 by CASP3 increases its kinase activity (in vitro).

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제품 프로토콜

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타겟 정보

Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration (PubMed : 15723050, PubMed : 9092543, PubMed : 9418861). Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2 (PubMed : 21457715). In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (PubMed : 18854160). Phosphorylates : PPP1R12A, LIMK1 and LIMK2 (PubMed : 11340065, PubMed : 11399775). May play a role in TFRC-mediated iron uptake (PubMed : 20188707). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity). Triggers the formation of an extrusion apical actin ring required for epithelial extrusion of apoptotic cells (PubMed : 29162624).
See full target information CDC42BPA

대체 명칭 보기

KIAA0451, CDC42BPA, Serine/threonine-protein kinase MRCK alpha, CDC42-binding protein kinase alpha, DMPK-like alpha, Myotonic dystrophy kinase-related CDC42-binding kinase alpha, MRCK alpha, Myotonic dystrophy protein kinase-like alpha
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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