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AB316081

Recombinant Human Claudin 18.2 (Active) protein

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Recombinant Human Claudin 18.2 (Active) protein is a Human Full Length protein, in the 1 to 261 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for ELISA, HPLC, FuncS.

대체 명칭 보기

UNQ778/PRO1572, CLDN18, Claudin-18

3 이미지
Functional Studies - Recombinant Human Claudin 18.2 (Active) protein (AB316081)
  • FuncS

Supplier Data

Functional Studies - Recombinant Human Claudin 18.2 (Active) protein (AB316081)

Human Claudin18.2 VLP captured on CM5 Chip via Anti-Claudin18.2 Antibody can bind Anti-Claudin18.2 Antibody with an affinity constant of 1.71 nM as determined in SPR assay (Biacore T200).

ELISA - Recombinant Human Claudin 18.2 (Active) protein (AB316081)
  • ELISA

Supplier Data

ELISA - Recombinant Human Claudin 18.2 (Active) protein (AB316081)

Immobilized Human Claudin 18.2 VLP at 5 μg/ml (100 μl/Well). Dose response curve for Anti Claudin 18.2 Antibody, hFc Tag with the EC50 of 7.5 ng/ml determined by ELISA (QC Test)

HPLC - Recombinant Human Claudin 18.2 (Active) protein (AB316081)
  • HPLC

Supplier Data

HPLC - Recombinant Human Claudin 18.2 (Active) protein (AB316081)

The purity of Human Claudin 18.2 VLP is greater than 95% as determined by SEC-HPLC.

주요 정보

Purity

>95% HPLC

Endotoxin 레벨

< 1 EU/µg

발현 시스템

HEK 293 cells

Tags

Tag free

Applications

ELISA, HPLC, FuncS

applications

Biologically active

Yes

Biological activity

Immobilized Human Claudin 18.2 VLP at 5ug/ml (100ul/Well). Dose response curve for Anti-Claudin 18.2 Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA.

Accession

Animal free

No

Carrier free

Yes

Species

Human

보관 버퍼

Constituents: PBS

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

  • 5
  • 1
Primary Antibodies

AB203563

Anti-Claudin18 antibody [34H14L15]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [34H14L15] (AB203563)

  • 5
  • 2
Proteins & Peptides

AB314537

Recombinant Macaca fascicularis Claudin18.2 protein - Active

Bioactive

Functional Studies - Recombinant Macaca fascicularis Claudin18.2 protein - Active (AB314537)

  • 0
  • 0
Proteins & Peptides

AB114374

Recombinant Human TROP2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human TROP2 protein (GST tag N-Terminus) (AB114374)

  • 0
  • 0
Proteins & Peptides

AB109839

Recombinant human ADA protein (His tag N-Terminus)

Bioactive

SDS-PAGE - Recombinant human ADA protein (His tag N-Terminus) (AB109839)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"5 µg/mL", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV","proteinLength":"Full Length","predictedMolecularWeight":"29 kDa","actualMolecularWeight":null,"aminoAcidEnd":261,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P56856","tags":[]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Claudin 18.2 also known as claudin 18 CLDN18 or claudin-18.2 is a tight junction protein with a molecular mass of approximately 27 kDa. It is primarily expressed in gastric epithelial cells and certain lung tissues. Claudin 18.2 is part of the claudin family which plays a critical role in forming tight junctions to regulate paracellular transport and maintain cellular polarity. This isoform specifically expressed in the stomach is encoded by the CLDN18 gene located on chromosome 3. Researchers often use techniques like Immunohistochemistry (IHC) referred to as claudin 18.2 IHC or cldn18 IHC to study its expression and localization.
Biological function summary

Claudin 18.2 functions within tight junction complexes creating a barrier and controlling the movement of ions and molecules between cells. It is vital for the integrity and function of epithelial cell barriers in the stomach. The protein interacts with other claudins and cytoplasmic proteins to form these tight junctions essential for maintaining tissue organization and permeability. Its unique expression pattern makes it a significant marker for gastric tissue and a potential target for therapeutics.

Pathways

Claudin 18.2 is involved in cellular pathways related to adhesion and barrier function. It plays a role in the tight junction pathway critical for epithelial barrier integrity. This pathway involves interactions with other claudin proteins and ZO-1 which is a cytoplasmic plaque protein that links claudins to the actin cytoskeleton reinforcing cellular structure. Another pathway where claudin 18.2 is important is the gastro-intestinal epithelium maintenance pathway which is fundamental for protecting gastric tissues.

Claudin 18.2 is a notable target in cancers especially gastric cancer. Its specific expression in gastric tissues makes it an attractive target for cancer immunotherapy as aberrant expression could indicate gastric cancer onset. Another disorder related to claudin 18.2 is asthma particularly where lung epithelial cells express this protein. Studies have identified links between claudin 18.2 and increased permeability in lung tissues implicating it in asthmatic pathophysiology. Its association with these conditions highlights the potential for claudin 18.2 as a diagnostic or therapeutic target in oncology and respiratory diseases.
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일반 정보

기능

Involved in alveolar fluid homeostasis via regulation of alveolar epithelial tight junction composition and therefore ion transport and solute permeability, potentially via downstream regulation of the actin cytoskeleton organization and beta-2-adrenergic signaling (By similarity). Required for lung alveolarization and maintenance of the paracellular alveolar epithelial barrier (By similarity). Acts to maintain epithelial progenitor cell proliferation and organ size, via regulation of YAP1 localization away from the nucleus and thereby restriction of YAP1 target gene transcription (By similarity). Acts as a negative regulator of RANKL-induced osteoclast differentiation, potentially via relocation of TJP2/ZO-2 away from the nucleus, subsequently involved in bone resorption in response to calcium deficiency (By similarity). Mediates the osteoprotective effects of estrogen, potentially via acting downstream of estrogen signaling independently of RANKL signaling pathways (By similarity).. Isoform A1. Involved in the maintenance of homeostasis of the alveolar microenvironment via regulation of pH and subsequent T-cell activation in the alveolar space, is therefore indirectly involved in limiting C.neoformans infection.. Isoform A2. Required for the formation of the gastric paracellular barrier via its role in tight junction formation, thereby involved in the response to gastric acidification.

서열 유사성

Belongs to the claudin family.

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제품 프로토콜

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타겟 정보

Involved in alveolar fluid homeostasis via regulation of alveolar epithelial tight junction composition and therefore ion transport and solute permeability, potentially via downstream regulation of the actin cytoskeleton organization and beta-2-adrenergic signaling (By similarity). Required for lung alveolarization and maintenance of the paracellular alveolar epithelial barrier (By similarity). Acts to maintain epithelial progenitor cell proliferation and organ size, via regulation of YAP1 localization away from the nucleus and thereby restriction of YAP1 target gene transcription (By similarity). Acts as a negative regulator of RANKL-induced osteoclast differentiation, potentially via relocation of TJP2/ZO-2 away from the nucleus, subsequently involved in bone resorption in response to calcium deficiency (By similarity). Mediates the osteoprotective effects of estrogen, potentially via acting downstream of estrogen signaling independently of RANKL signaling pathways (By similarity).. Isoform A1. Involved in the maintenance of homeostasis of the alveolar microenvironment via regulation of pH and subsequent T-cell activation in the alveolar space, is therefore indirectly involved in limiting C.neoformans infection.. Isoform A2. Required for the formation of the gastric paracellular barrier via its role in tight junction formation, thereby involved in the response to gastric acidification.
See full target information CLDN18
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Product promise

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