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AB265857

Recombinant human Cystatin A protein (Active) (His tag N-Terminus)

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Recombinant human Cystatin A protein (Active) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 98 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS, Mass Spec.

대체 명칭 보기

STF1, STFA, CSTA, Cystatin-A, Cystatin-AS, Stefin-A

2 이미지
Functional Studies - Recombinant human Cystatin A protein (Active) (His tag N-Terminus) (AB265857)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Cystatin A protein (Active) (His tag N-Terminus) (AB265857)

Functional analysis of ab265857.

The IC50 value is < 1.0nM. The inhibitory function of Cystatin A on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25°C.

SDS-PAGE - Recombinant human Cystatin A protein (Active) (His tag N-Terminus) (AB265857)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Cystatin A protein (Active) (His tag N-Terminus) (AB265857)

3µg by SDS-PAGE under reducing condition and visualized by coomassie blue stain

주요 정보

Purity

>95% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, Mass Spec, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The IC50 value is < 1.0nM. The inhibitory function of Cystatin A on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25°C.

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMIPGGLSEAKPATPEIQEIVDKVKPQLEEKTNETYGKLEAVQYKTQVVAGTNYYIKVRAGDNKYMHLKVFKSLPGQNEDLVLTGYQVDKNKDDELTGF","proteinLength":"Full Length","predictedMolecularWeight":"13.1 kDa","actualMolecularWeight":"13.1 kDa","aminoAcidEnd":98,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P01040","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True

일반 정보

기능

This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.

서열 유사성

Belongs to the cystatin family.

제품 프로토콜

타겟 정보

This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.
See full target information CSTA

Product promise

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