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AB324403

Recombinant Human Cytokeratin 18 Fragment (CK18-30) Protein Standard

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Recombinant Human Cytokeratin 18 Fragment (CK18-30) Protein Standard is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.
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Sandwich ELISA - Recombinant Human Cytokeratin 18 Fragment (CK18-30) Protein Standard (AB324403)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Cytokeratin 18 Fragment (CK18-30) Protein Standard (AB324403)

Sandwich ELISA with the capture antibody ab244688 dilution at 2 μg/mL and detector antibody ab259742 dilution at 0.5 μg/mL after biotin conjugation.

SDS-PAGE - Recombinant Human Cytokeratin 18 Fragment (CK18-30) Protein Standard (AB324403)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Cytokeratin 18 Fragment (CK18-30) Protein Standard (AB324403)

SDS-PAGE analysis of ab324403 under reducing conditions for 2ug protein.

주요 정보

Purity

>80% SDS-PAGE

발현 시스템

HEK 293 cells

Tags

Tag free

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

P05783-1

Animal free

Yes

Carrier free

No

Species

Human

보관 버퍼

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

  • 0
  • 0
Assay Kits

AB256747

Human Cytokeratin 18 Fragment (CK18-30) (Asp396) Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human Cytokeratin 18 Fragment (CK18-30) (Asp396) Antibody Pair - BSA and Azide free (AB256747)

  • 0
  • 0
Primary Antibodies

AB244688

Anti-Cytokeratin 18 antibody [EPR20619-8] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Cytokeratin 18 antibody [EPR20619-8] - BSA and Azide free (Capture) (AB244688)

  • 0
  • 0
Assay Kits

AB254515

Human Cytokeratin 18 Fragment ELISA Kit (CK18-30)

Recombinant|SimpleStep

ELISA - Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) (AB254515)

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Proteins & Peptides

AB156970

Recombinant Human Cytokeratin 8 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Cytokeratin 8 protein (His tag N-Terminus) (AB156970)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

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서열 정보

[{"linker":null,"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"37.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P05783","tags":[]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification His Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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제품 프로토콜

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타겟 정보

Required for the formation of KRT8/KRT18 filaments that are involved in ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement (PubMed : 26823019). Also acts downstream of ROCK kinase activation as part of a positive feedback mechanism in response to cellular mechanical stress loading (PubMed : 26823019). Organization and orientation of KRT18 filaments are responsible for the properly elongated morphology of epithelial tubules (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
See full target information KRT18

대체 명칭 보기

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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