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AB87755

Recombinant human Dihydrofolate reductase (DHFR) protein (His tag N-Terminus)

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Recombinant human Dihydrofolate reductase (DHFR) protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 187 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.
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SDS-PAGE - Recombinant human Dihydrofolate reductase (DHFR) protein (His tag N-Terminus) (AB87755)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Dihydrofolate reductase (DHFR) protein (His tag N-Terminus) (AB87755)

ab87755 at 3 ug visualised by SDS-PAGE. Observed band size is approximately 27 kDa.

주요 정보

Purity

>95% SDS-PAGE

purified by using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific activity is > 2000pmol/min/μg, and is defined as the amount of enzyme that converts 1.0pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25°C.

Accession

P00374-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB234592

Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)

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Assay Kits

AB239705

Dihydrofolate Reductase Assay Kit (Colorimetric)

Functional Studies - Dihydrofolate Reductase Assay Kit (Colorimetric) (AB239705)

  • 0
  • 0
Primary Antibodies

AB124814

Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (AB124814)

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  • 1
Primary Antibodies

AB279584

APC Anti-CD32A antibody [02]

Flow Cytometry - APC Anti-CD32A antibody [02] (AB279584)

  • 0
  • 0
Assay Kits

AB288923

Human Thymidylate Synthase ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Thymidylate Synthase ELISA Kit (AB288923)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND","proteinLength":"Full Length","predictedMolecularWeight":"23.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":187,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00374","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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일반 정보

기능

Catalyzes the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate in a NADPH-dependent manner (PubMed : 12096917, PubMed : 15039552, PubMed : 17569517, PubMed : 19196009, PubMed : 19478082, PubMed : 21876184, PubMed : 9719595). Key enzyme in folate metabolism. Contributes to the nuclear and mitochondrial de novo thymidylate biosynthesis pathway (PubMed : 21876188, PubMed : 22235121). Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

서열 유사성

Belongs to the dihydrofolate reductase family.

Post-translational modifications

Sumoylated by UBE2I/UBC9.

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제품 프로토콜

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타겟 정보

Catalyzes the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate in a NADPH-dependent manner (PubMed : 12096917, PubMed : 15039552, PubMed : 17569517, PubMed : 19196009, PubMed : 19478082, PubMed : 21876184, PubMed : 9719595). Key enzyme in folate metabolism. Contributes to the nuclear and mitochondrial de novo thymidylate biosynthesis pathway (PubMed : 21876188, PubMed : 22235121). Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
See full target information Dihydrofolate reductase

대체 명칭 보기

Dihydrofolate reductase, DHFR
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Product promise

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