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AB176956

Recombinant human DNA Polymerase Kappa/POLK protein

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Recombinant human DNA Polymerase Kappa/POLK protein is a Human Fragment protein, in the 1 to 560 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, ELISA, WB, FuncS.

대체 명칭 보기

DINB1, POLK, DNA polymerase kappa, DINB protein, DINP

2 이미지
Functional Studies - Recombinant human DNA Polymerase Kappa/POLK protein (AB176956)
  • FuncS

Supplier Data

Functional Studies - Recombinant human DNA Polymerase Kappa/POLK protein (AB176956)

DNA polymerizing activity of ab176956
50 mM Tris-HCl pH 8.0, 2 mM MgCl2, 1 mM DTT, 0.1 mg / ml BSA, 0.1 mM dNTP, 100 nM primer/template(13-mer/30-mer), DNA polymerase kappa/POLK at 13~50 ng/25μl (lanes 1,2, and 3 respectiviely) per assay, incubated at 37℃ for 10 min.

SDS-PAGE - Recombinant human DNA Polymerase Kappa/POLK protein (AB176956)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human DNA Polymerase Kappa/POLK protein (AB176956)

SDS-PAGE analysis of ab196956.

주요 정보

Purity

>90% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag C-Terminus

Applications

WB, SDS-PAGE, FuncS, ELISA

applications

Biologically active

Yes

Biological activity

Active

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 6 - 8.5 Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.14% Sodium phosphate

storage-buffer

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보완 제품

Proteins & Peptides

AB132162

Recombinant Human SMUG1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human SMUG1 protein (GST tag N-Terminus) (AB132162)

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  • 0
Primary Antibodies

AB86076

Anti-DNA Polymerase Kappa/POLK antibody

Western blot - Anti-DNA Polymerase Kappa/POLK antibody (AB86076)

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Primary Antibodies

AB76949

Anti-Strep-tag II antibody

Lab Essentials

Western blot - Anti-Strep-tag II antibody (AB76949)

  • 4
  • 7
Primary Antibodies

AB307676

Anti-Strep-tag II antibody [EPR28119-43]

BOND RX™ Validated|Recombinant|Lab Essentials|20ul selling size|RabMAb

Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [EPR28119-43] (AB307676)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISPLLFEESPSDVQPPGDPFQVNFEEQNNPQILQNSVVFGTSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKERLKGRTVTIKLKNVNFEVKTRASTVSSVVSTAEEIFAIAKELLKTEIDADFPHPLRLRLMGVRISSFPNEEDRKHQQRSIIGFLQAGNQALSATECTLEKTDKDKFVKPLE","proteinLength":"Fragment","predictedMolecularWeight":"64 kDa","actualMolecularWeight":null,"aminoAcidEnd":560,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UBT6","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

DNA polymerase kappa also known as POLK or kappa polymerase is a specialized enzyme involved in DNA replication and repair. This protein weighs approximately 93 kDa. POLK is expressed across various human tissues with notable activity in testis and placenta. Mechanically DNA polymerase kappa participates in translesion synthesis a process that permits DNA replication to proceed past damaged or abnormal bases allowing cells to overcome blockages that conventional DNA polymerases cannot bypass.
Biological function summary

DNA polymerase kappa plays a significant role in error-prone repair mechanisms contributing to DNA damage tolerance. It is not known to associate directly with a larger complex but works in concert with other translesion synthesis polymerases such as DNA polymerase eta. Through its action POLK introduces nucleotides opposite DNA lesions maintaining replication fork progression and cell viability under genotoxic stress conditions.

Pathways

DNA polymerase kappa is integral to the translesion synthesis pathway and participates in the broader DNA damage response system. It relates functionally to other DNA polymerases including polymerase eta and zeta which together modulate cellular responses to DNA damage and repair. The interplay between these enzymes facilitates flexible and adaptive responses to various genotoxic insults highlighting their roles in maintaining genomic stability.

DNA polymerase kappa's association with carcinogenesis is of particular interest. Its error-prone activity can result in mutagenesis linking it to cancer development. POLK's expression or malfunction influences conditions like xeroderma pigmentosum where defective DNA repair increases cancer risk. Connections with the p53 pathway illustrate that defects in related DNA repair proteins can exacerbate the development of neoplastic diseases highlighting considerable implications for targeted cancer therapies.
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일반 정보

기능

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.

서열 유사성

Belongs to the DNA polymerase type-Y family.

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제품 프로토콜

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타겟 정보

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
See full target information POLK
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Product promise

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