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AB238220

Recombinant Human DNase gamma protein (Tagged)

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Recombinant Human DNase gamma protein (Tagged) is a Human Full Length protein, in the 21 to 305 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

대체 명칭 보기

DHP2, DNAS1L3, DNASE1L3, Deoxyribonuclease gamma, DNase gamma, DNase I homolog protein DHP2, Deoxyribonuclease I-like 3, Liver and spleen DNase, DNase I-like 3, LS-DNase, LSD

1 이미지
SDS-PAGE - Recombinant Human DNase gamma protein (Tagged) (AB238220)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human DNase gamma protein (Tagged) (AB238220)

ab238220 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

주요 정보

Purity

>90% SDS-PAGE

발현 시스템

Escherichia coli

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

  • 5
  • 1
Proteins & Peptides

AB275555

Recombinant human DNase I protein (Active)

Functional Studies - Recombinant human DNase I protein (Active) (AB275555)

  • 0
  • 0
Primary Antibodies

AB309566

Anti-DNase I antibody [EPR25941-19] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-DNase I antibody [EPR25941-19] - BSA and Azide free (Detector) (AB309566)

  • 0
  • 0
Primary Antibodies

AB52771

Anti-YAP1 antibody [EP1674Y]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-YAP1 antibody [EP1674Y] (AB52771)

  • 4
  • 16
Primary Antibodies

AB244732

Anti-DR5 antibody [EPR21135-223] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-DR5 antibody [EPR21135-223] - BSA and Azide free (Capture) (AB244732)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MRICSFNVRSFGESKQEDKNAMDVIVKVIKRCDIILVMEIKDSNNRICPILMEKLNRNSRRGITYNYVISSRLGRNTYKEQYAFLYKEKLVSVKRSYHYHDYQDGDADVFSREPFVVWFQSPHTAVKDFVIIPLHTTPETSVKEIDELVEVYTDVKHRWKAENFIFMGDFNAGCSYVPKKAWKNIRLRTDPRFVWLIGDQEDTTVKKSTNCAYDRIVLRGQEIVSSVVPKSNSVFDFQKAYKLTEEEALDVSDHFPVEFKLQSSRAFTNSKKSVTLRKKTKSKRS","proteinLength":"Full Length","predictedMolecularWeight":"60.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":305,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q13609","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
False
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일반 정보

기능

Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends (By similarity). Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA (PubMed : 10807908, PubMed : 14646506, PubMed : 27293190, PubMed : 9070308, PubMed : 9714828). Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis (PubMed : 23229555, PubMed : 24312463). The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells (By similarity). Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity (PubMed : 27293190). Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).

서열 유사성

Belongs to the DNase I family.

Post-translational modifications

Poly-ADP-ribosylated by PARP1. ADP-ribosylation negatively regulates enzymatic activity during apoptosis.

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends (By similarity). Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA (PubMed : 10807908, PubMed : 14646506, PubMed : 27293190, PubMed : 9070308, PubMed : 9714828). Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis (PubMed : 23229555, PubMed : 24312463). The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells (By similarity). Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity (PubMed : 27293190). Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).
See full target information DNASE1L3
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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