Recombinant human DNase I protein (Active) is a Human Fragment protein, in the 19 to 259 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS, WB.
대체 명칭 보기
DNL1, DRNI, DNASE1, Deoxyribonuclease-1, Deoxyribonuclease I, DNase I
- FuncS
Supplier Data
Functional Studies - Recombinant human DNase I protein (Active) (AB275555)
The binding activity of DNase I and ACTb.
DNase I were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-DNase I pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human DNase I protein (Active) (AB275555)
SDS-PAGE - Recombinant human DNase I protein (Active) (ab275555).
보완 제품
Reagents, Controls & Accessories
AB116029
Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)
- 5
- 1
Primary Antibodies
AB309566
Anti-DNase I antibody [EPR25941-19] - BSA and Azide free (Detector)
RabMAb|Recombinant
![Sandwich ELISA - Anti-DNase I antibody [EPR25941-19] - BSA and Azide free (Detector) (AB309566)](https://content.abcam.com/products/images/dnase-i-antibody-epr25941-19-bsa-and-azide-free-detector-ab309566--sandwich-elisa-img416810.jpg)
- 0
- 0
Reactivity 정보
서열 정보
특성 및 보관 정보
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일반 정보
기능
Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed : 11241278, PubMed : 2251263, PubMed : 2277032). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis (PubMed : 11241278). Binds specifically to G-actin and blocks actin polymerization (By similarity). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).
서열 유사성
Belongs to the DNase I family.
타겟 정보
Product promise
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