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AB241444

Recombinant Human DOCK8 protein (His tag)

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Recombinant Human DOCK8 protein (His tag) is a Human Fragment protein, in the 560 to 729 aa range, expressed in Yeast, with >85%, suitable for SDS-PAGE.

대체 명칭 보기

Dedicator of cytokinesis protein 8, DOCK8

1 이미지
SDS-PAGE - Recombinant Human DOCK8 protein (His tag) (AB241444)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human DOCK8 protein (His tag) (AB241444)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab241444.

주요 정보

Purity

>85% SDS-PAGE

발현 시스템

Yeast

Tags

10x His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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보완 제품

Primary Antibodies

AB175208

Anti-DOCK8 antibody [EPR12511]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (AB175208)

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Proteins & Peptides

AB95395

Recombinant Human ketohexokinase protein (Tag Free)

SDS-PAGE - Recombinant Human ketohexokinase protein (Tag Free) (AB95395)

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  • 0
Proteins & Peptides

AB116891

Recombinant Human Blood Group Kell Antigen protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Blood Group Kell Antigen protein (GST tag N-Terminus) (AB116891)

  • 0
  • 0
Primary Antibodies

AB211777

Anti-NR2F2 antibody [EPR18443]

RabMAb|Recombinant|Advanced Validation|20ul selling size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [EPR18443] (AB211777)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"RNLLYVYPQRLNFVNKLASARNITIKIQFMCGEDASNAMPVIFGKSSGPEFLQEVYTAVTYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASVETLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSMHSAEKVPLQNPPIKWAEGHKGVFNIEVQAV","proteinLength":"Fragment","predictedMolecularWeight":"21.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":729,"aminoAcidStart":560,"nature":"Recombinant","expressionSystem":"Yeast","accessionNumber":"Q8NF50","tags":[{"tag":"10x His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

DOCK8 also known as Dedicator of Cytokinesis 8 weighs approximately 210 kDa. It acts mechanically as a guanine nucleotide exchange factor (GEF) for small Rho GTPases which help regulate actin cytoskeleton dynamics. DOCK8 is expressed widely particularly in hematopoietic cells such as lymphocytes. It also associates with cell membranes where it facilitates signaling pathways involved in immune cell function.
Biological function summary

DOCK8 actively participates in immune system regulation by impacting the cytoskeletal rearrangements necessary for proper signaling and movement of immune cells. DOCK8 engages with proteins like DOCK2 and DOCK3 forming a complex that modulates these cellular mechanisms. Its function enhances the proper survival and immune response activities of leukocytes assisting in immune surveillance and pathogen clearance.

Pathways

Several immune-related signaling pathways involve DOCK8 especially the Rac-dependent pathways that affect actin polymerization. DOCK8 interacts closely with the CRK-like protein (CRKL) to mediate these processes. These pathways highlight the interaction between DOCK8 and other proteins critical to immune cell function positioning DOCK8 as an important regulator in immune system pathways.

Mutations in the DOCK8 gene correlate with DOCK8 deficiency a form of combined immunodeficiency characterized by eczema recurrent infections and high risk for malignancies. This disorder directly connects DOCK8 with suboptimal immune function. Furthermore DOCK8's interaction with the WASP protein plays a role in disrupting immune system health contributing to the pathophysiology of this immunodeficiency.
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일반 정보

기능

Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP (PubMed : 22461490, PubMed : 28028151). During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane (PubMed : 28028151). Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (PubMed : 25762780).

서열 유사성

Belongs to the DOCK family.

Post-translational modifications

In response to chemokine CXCL12/SDF-1-alpha stimulation, phosphorylated by PRKCA/PKC-alpha which promotes DOCK8 dissociation from LRCH1.

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제품 프로토콜

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타겟 정보

Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP (PubMed : 22461490, PubMed : 28028151). During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane (PubMed : 28028151). Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (PubMed : 25762780).
See full target information DOCK8
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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