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AB290068

Recombinant Human DPP4 Protein (Active)

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Recombinant Human DPP4 Protein (Active) is a Human Fragment protein, in the 29 to 766 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for FuncS.

대체 명칭 보기

CD26, ADCP2, DPP4, Dipeptidyl peptidase 4, ADABP, Adenosine deaminase complexing protein 2, Dipeptidyl peptidase IV, T-cell activation antigen CD26, TP103, ADCP-2, DPP IV

2 이미지
Functional Studies - Recombinant Human DPP4 Protein (Active) (AB290068)
  • FuncS

Supplier Data

Functional Studies - Recombinant Human DPP4 Protein (Active) (AB290068)

Fully biologically active determined by its ability to cleave fluorogenic peptide substrate H-Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). Specific activity is ≥ 8900 pmol/min/ug.

SDS-PAGE - Recombinant Human DPP4 Protein (Active) (AB290068)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human DPP4 Protein (Active) (AB290068)

SDS-PAGE analysis of ab290068

주요 정보

Purity

>95% HPLC

Endotoxin 레벨

<0.005 EU/µg

발현 시스템

HEK 293 cells

Tags

Tag free

Applications

FuncS

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by its ability to cleave fluorogenic peptide substrate H-Gly-Pro-7-amido-4-methylcoumarin (GP-AMC).
Specific activity is ≥ 8900 pmol/min/ug.

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325997

Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color) (AB325997)

  • 0
  • 0
Assay Kits

AB204722

Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric)

Functional Studies - Dipeptidyl peptidase IV (DPP4) Activity Assay Kit (Fluorometric) (AB204722)

  • 0
  • 0
Primary Antibodies

AB91171

APC Anti-DPP4 antibody [TP1/19]

Flow Cytometry - APC Anti-DPP4 antibody [TP1/19] (AB91171)

  • 0
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Assay Kits

AB184857

Human GLP1 (7-36) ELISA Kit

Recombinant|SimpleStep

ELISA - Human GLP1 (7-36) ELISA Kit (AB184857)

  • 5
  • 3
Assay Kits

AB252365

Human DPP4 (CD26) ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (AB252365)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"NKGTDDATADSRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENNILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQFILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIPNNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRITWTGKEDIIYNGITDWVYEEEVFSAYSALWWSPNGTFLAYAQFNDTEVPLIEYSFYSDESLQYPKTVRVPYPKAGAVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGDHYLCDVTWATQERISLQWLRRIQNYSVMDICDYDESSGRWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSFYKIISNEEGYRHICYFQIDKKDCTFITKGTWEVIGIEALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCLSCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTLHSSVNDKGLRVLEDNSALDKMLQNVQMPSKKLDFIILNETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADTVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDVGVDFQAMWYTDEDHGIASSTAHQHIYTHMSHFIKQCFSLP","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":766,"aminoAcidStart":29,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P27487","tags":[]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Dipeptidyl peptidase 4 also known as DPP4 or CD26 functions as a serine exopeptidase. This enzyme is involved in cleaving dipeptides from the N-terminus of polypeptides. DPP4 with a mass of approximately 110 kDa is expressed in various tissues such as the liver intestines kidney and lymphocytes. It exists in both a membrane-bound form and a soluble form in blood plasma.
Biological function summary

DPP4 plays a significant role in glucose metabolism and immune function. The enzyme interacts with members of the immune system and regulates proteins such as chemokines growth factors and neuropeptides. DPP4 acts independently and is not known to be part of a larger complex. It modulates biological processes by inactivating peptide hormones and signaling molecules which impacts glucose homeostasis and immune responses.

Pathways

DPP4 impacts the incretin signaling pathway and the immune response pathway. Incretin hormones like glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are substrates of DPP4 and play important roles in glucose metabolism. DPP4 also interacts with proteins such as adenosine deaminase (ADA) which impacts T-cell function and immune regulation. The regulation of these pathways by DPP4 influences metabolic and immune health.

DPP4 involvement is relevant in type 2 diabetes and immunological disorders. In type 2 diabetes DPP4 inhibitors modulate the incretin hormones improving glycemic control. Proteins like GLP-1 are affected by DPP4 activity connecting it to diabetes management. In autoimmune diseases altered DPP4 expression can impact immune cell functions influencing conditions such as rheumatoid arthritis. Through its effects on ADA and associated pathways DPP4 can influence both metabolic and immune-related disorders.
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일반 정보

기능

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation (PubMed : 10900005, PubMed : 10951221, PubMed : 11772392, PubMed : 17287217). Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC (PubMed : 10900005, PubMed : 10951221, PubMed : 11772392, PubMed : 14691230). Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed : 17287217). Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion (PubMed : 11772392). In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM (PubMed : 10593948, PubMed : 16651416). May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation (PubMed : 18708048). When overexpressed, enhanced cell proliferation, a process inhibited by GPC3 (PubMed : 17549790). Also acts as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones such as brain natriuretic peptide 32 (PubMed : 10570924, PubMed : 16254193). Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline (PubMed : 10593948).. (Microbial infection) Acts as a receptor for human coronavirus MERS-CoV-2.

서열 유사성

Belongs to the peptidase S9B family. DPPIV subfamily.

Post-translational modifications

The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.. N- and O-Glycosylated.. Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.

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제품 프로토콜

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타겟 정보

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation (PubMed : 10900005, PubMed : 10951221, PubMed : 11772392, PubMed : 17287217). Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC (PubMed : 10900005, PubMed : 10951221, PubMed : 11772392, PubMed : 14691230). Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed : 17287217). Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion (PubMed : 11772392). In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM (PubMed : 10593948, PubMed : 16651416). May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation (PubMed : 18708048). When overexpressed, enhanced cell proliferation, a process inhibited by GPC3 (PubMed : 17549790). Also acts as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones such as brain natriuretic peptide 32 (PubMed : 10570924, PubMed : 16254193). Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline (PubMed : 10593948).. (Microbial infection) Acts as a receptor for human coronavirus MERS-CoV-2.
See full target information DPP4
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Product promise

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