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AB137172

Recombinant Human DTD1 protein (His tag N-Terminus)

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Recombinant Human DTD1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 209 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

C20orf88, DUEB, HARS2, DTD1, D-aminoacyl-tRNA deacylase 1, DTD, DNA-unwinding element-binding protein B, Gly-tRNA(Ala) deacylase, Histidyl-tRNA synthase-related, DUE-B

1 이미지
SDS-PAGE - Recombinant Human DTD1 protein (His tag N-Terminus) (AB137172)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human DTD1 protein (His tag N-Terminus) (AB137172)

15% SDS-PAGE analysis of ab137172 (3μg)

주요 정보

Purity

>85% SDS-PAGE

ab137172 was purified using conventional chromatography.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Cell Lines & Lysates

AB258539

Human NPM3 knockout HEK-293T cell lysate

Sanger Sequencing - Human NPM3 knockout HEK-293T cell lysate (AB258539)

  • 0
  • 0
Primary Antibodies

AB198224

Anti-NPM3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPM3 antibody (AB198224)

  • 1
  • 1
Primary Antibodies

AB140632

Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470] (AB140632)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMKAVVQRVTRASVTVGGEQISAIGRGICVLLGISLEDTQKELEHMVRKILNLRVFEDESGKHWSKSVMDKQYEILCVSQFTLQCVLKGNKPDFHLAMPTEQAEGFYNSFLEQLRKTYRPELIKDGKFGAYMQVHIQNDGPVTIELESPAPGTATSDPKQLSKLEKQQQRKEKTRAKGPSESSKERNTPRKEDRSASSGAEGDVSSEREP","proteinLength":"Full Length","predictedMolecularWeight":"25.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":209,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8TEA8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

DTD1 also known as D-Tyr-tRNA deacylase is a protein involved mechanically in detoxifying D-amino acids that mistakenly attach to tRNA molecules. It functions by hydrolyzing the ester bond between the D-amino acid and tRNA preventing incorporation during protein synthesis. The protein has a relatively small mass of approximately 20 kilodaltons. DTD1 is expressed in various tissues with notable expression in the liver and kidney where clearing abnormal peptides is critical.
Biological function summary

The removal of incorrect D-amino acids ensures fidelity in protein translation. DTD1 functions not only as a single enzyme but associates with other molecules in multi-enzyme complexes. These complexes help maintain the accuracy of protein biosynthesis across different cellular environments. By doing so DTD1 contributes to cellular homeostasis and conserves the correct folding and function of proteins necessary for normal cellular activity.

Pathways

DTD1 plays a significant role in aminoacyl-tRNA biosynthesis and interaction pathways. It collaborates with proteins like tRNA synthetases in the aminoacyl-tRNA synthesis pathway ensuring only L-amino acids are incorporated into growing polypeptide chains. This pathway is essential for high-fidelity protein translation which allows cells to synthesize functional proteins efficiently affecting overall protein homeostasis.

Mismanagement of D-amino acids can lead to neurodegenerative diseases and metabolic disorders. For example abnormal D-amino acid levels have been linked to disorders like Alzheimer's disease. In such conditions DTD1's activity or lack thereof can influence the progression of the disease. Additionally interactions with other related proteins like L-amino acid oxidase can impact disease pathways as imbalances in these systems contribute to disease states.
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일반 정보

기능

Possible ATPase (PubMed : 15653697) involved in DNA replication, may facilitate loading of CDC45 onto pre-replication complexes (PubMed : 20065034).. An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.

서열 유사성

Belongs to the DTD family.

Post-translational modifications

Preferentially phosphorylated in cells arrested early in S phase (PubMed:15653697). Phosphorylation in the C-terminus weakens the interaction with CDC45 (PubMed:20065034).

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

Possible ATPase (PubMed : 15653697) involved in DNA replication, may facilitate loading of CDC45 onto pre-replication complexes (PubMed : 20065034).. An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.
See full target information DTD1
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Product promise

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