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AB156723

Recombinant Human EED protein (His tag N-Terminus)

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Recombinant Human EED protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 441 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

대체 명칭 보기

Polycomb protein EED, hEED, Embryonic ectoderm development protein, WD protein associating with integrin cytoplasmic tails 1, WAIT-1, EED

1 이미지
SDS-PAGE - Recombinant Human EED protein (His tag N-Terminus) (AB156723)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human EED protein (His tag N-Terminus) (AB156723)

15% SDS-PAGE analysis of ab156723 (3μg).

주요 정보

Purity

>85% SDS-PAGE

ab156723 is purified using conventional chromatography techniques

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Primary Antibodies

AB240650

Anti-EED antibody [EPR23043-5] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated|Trial Size

Immunoprecipitation - Anti-EED antibody [EPR23043-5] - ChIP Grade (AB240650)

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Proteins & Peptides

AB70789

Recombinant human Lyn protein (GST tag N-Terminus)

Functional Studies - Recombinant human Lyn protein (AB70789)

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Proteins & Peptides

AB84696

Recombinant human Fyn protein (GST tag N-Terminus)

Functional Studies - Recombinant human Fyn protein (AB84696)

  • 0
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Sample Prep & Detection Kits

AB102890

HRP Conjugation Kit - Lightning-Link®

Conjugation - HRP Conjugation Kit - Lightning-Link® (AB102890)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR","proteinLength":"Full Length","predictedMolecularWeight":"52.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":441,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O75530","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The EED protein also known as embryonic ectoderm development plays an important mechanical role within the cell. It is a component of the polycomb repressive complex 2 (PRC2) that mediates transcriptional silencing of target genes. The molecular weight of EED protein is approximately 50 kDa. EED is expressed in various tissues and is a critical mediator in maintaining transcriptional repression necessary for embryonic stem cell identity and differentiation.
Biological function summary

EED functions as a repressor of gene transcription through the polycomb group complex. It interacts with other components of PRC2 such as EZH2 SUZ12 to establish and maintain chromatin modifications. EED acts as a scaffold allowing communication between other proteins essential in the assembly and function of PRC2. These interactions regulate histone methylation contributing to gene silencing.

Pathways

EED plays a central role in the regulation of the Wnt and Hedgehog pathways. These pathways are critical for controlling cell fate decisions and developmental processes. In the Wnt pathway EED in conjunction with other PRC2 members modulates β-catenin-mediated transcription. In the Hedgehog pathway EED impacts the Gli family of transcription factors underlining its importance in development and cellular proliferation regulation.

EED has been linked to various cancers and developmental disorders. Aberrant EED function and localization relate to overactivation of the Wnt pathway an aspect seen in colorectal cancer. EED also interacts with EHZ2 contributing to tumorigenesis through unchecked cell division and proliferation. In the context of Weaver syndrome mutations within EED are involved consolidating its important association with developmental anomalies.
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일반 정보

기능

Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.

서열 유사성

Belongs to the WD repeat ESC family.

Post-translational modifications

Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines.

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제품 프로토콜

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타겟 정보

Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
See full target information EED
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Product promise

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