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AB104755

Recombinant Human EEF1D protein (His tag N-Terminus)

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Recombinant Human EEF1D protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 281 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

EF1D, EEF1D, Elongation factor 1-delta, EF-1-delta, Antigen NY-CO-4

1 이미지
SDS-PAGE - Recombinant Human EEF1D protein (His tag N-Terminus) (AB104755)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human EEF1D protein (His tag N-Terminus) (AB104755)

15% SDS-PAGE (3μg).
Note : Molecular weight on SDS-PAGE will appear higher than predicted.

주요 정보

Purity

>90% SDS-PAGE

ab104755 is purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Primary Antibodies

AB153728

Anti-EEF1D antibody

Immunocytochemistry/ Immunofluorescence - Anti-EEF1D antibody (AB153728)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNKI","proteinLength":"Full Length","predictedMolecularWeight":"33.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":281,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P29692","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EEF1D also known as elongation factor 1-delta plays an important role in protein synthesis by facilitating the delivery of aminoacyl-tRNA to the ribosome A-site during translation elongation. The protein has a molecular mass of approximately 33 kDa. EEF1D is expressed in various tissues with notable presence in the heart and skeletal muscle. This expression pattern suggests its involvement in tissue-specific synthesis of proteins needed for diverse cellular functions.
Biological function summary

EEF1D interacts with other components of the translational machinery to form the elongation factor complex which is essential for efficient protein biosynthesis. EEF1D's interaction with the EF1B complex supports the recycling of guanine nucleotides which prepares EF1A for reuse in additional rounds of protein elongation. This collaboration within the machinery highlights EEF1D's importance in maintaining proper protein synthesis rates which are important for cellular growth and repair processes.

Pathways

EEF1D is integral in the translation and protein metabolism pathways. The protein's roles align closely with those of EEF1A and EEF1B ensuring smooth continuation of the elongation phase of protein synthesis. Its participation in these pathways highlights how alterations in EEF1D function might disrupt broader cellular processes impacting how cells respond to genetic instructions and manage protein construction workflows.

EEF1D has connections to cancer and neurodegenerative diseases. Abnormal expression or mutations in EEF1D can influence tumor progression by altering protein synthesis rates or pathways related to cell proliferation. Additionally connections exist between EEF1D and EEF1A2 in neurodegenerative conditions where disruptions in protein synthesis contribute to neuronal damage and disease manifestation. Understanding EEF1D's role in these conditions may offer insight into potential therapeutic targets for intervention.
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일반 정보

기능

Isoform 1. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.. Isoform 2. Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).

서열 유사성

Belongs to the EF-1-beta/EF-1-delta family.

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제품 프로토콜

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타겟 정보

Isoform 1. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.. Isoform 2. Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).
See full target information EEF1D
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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