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AB60007

Recombinant Human eIF4EBP1 protein (His tag N-Terminus)

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(1 리뷰)

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(0 출판물)

Recombinant Human eIF4EBP1 protein (His tag N-Terminus) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, WB.
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Western blot - Recombinant Human eIF4EBP1 protein (His tag N-Terminus) (AB60007)
  • WB

Unknown

Western blot - Recombinant Human eIF4EBP1 protein (His tag N-Terminus) (AB60007)

All lanes:

Western blot - Anti-eIF4EBP1 antibody (<a href='/ko/products/primary-antibodies/eif4ebp1-antibody-ab2606'>ab2606</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human eIF4EBP1 protein (His tag N-Terminus) (ab60007) at 0.01 µg

Lane 2:

Western blot - Recombinant Human eIF4EBP1 protein (His tag N-Terminus) (ab60007) at 0.001 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 13 kDa

true

Exposure time: 10s

SDS-PAGE - Recombinant Human eIF4EBP1 protein (AB60007)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human eIF4EBP1 protein (AB60007)

ab60007 on SDS-PAGE.

주요 정보

Purity

>95% Densitometry

Purified via His tag. Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

His tag N-Terminus

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

Q13541-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.00308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

  • 0
  • 0
Proteins & Peptides

AB195250

Recombinant Human RPS6 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human RPS6 protein (GST tag N-Terminus) (AB195250)

  • 0
  • 0
Primary Antibodies

AB32024

Anti-eIF4EBP1 antibody [Y329]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-eIF4EBP1 antibody [Y329] (AB32024)

  • 0
  • 0
Primary Antibodies

AB126730

Anti-DDX5 antibody [EPR7239]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-DDX5 antibody [EPR7239] (AB126730)

  • 5
  • 2
Primary Antibodies

AB52922

Anti-HLA A antibody [EP1395Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-HLA A antibody [EP1395Y] (AB52922)

  • 4
  • 15
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab60007 can be used as a WB positive control in conjunction with <a href='/ko/products/primary-antibodies/eif4ebp1-antibody-ab2606'>ab2606</a>.</p>" } } }
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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q13541","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification His Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EIF4EBP1 also known as 4EBP1 or p4EBP1 is an important protein that acts as a translational repressor by binding to eIF4E therefore inhibiting cap-dependent translation. The molecular weight of 4EBP1 is approximately 12 kDa. This protein is ubiquitously expressed in various tissues indicating its widespread involvement in cellular functions. eIF4EBP1 can be detected and quantified using techniques like the eIF4EBP1 ELISA making it a common target in research for its role in translation regulation.
Biological function summary

EIF4EBP1 plays an important role in regulating cell growth and proliferation by modulating protein synthesis. It is a part of the eIF4F complex which is responsible for the initiation of mRNA translation. When hypophosphorylated eIF4EBP1 binds tightly to eIF4E and prevents the assembly of the active eIF4F complex leading to reduced translation initiation of mRNAs involved in growth and survival. This regulation is important in conditions where cells need to adapt to metabolic stress or external signals.

Pathways

The mammalian target of rapamycin (mTOR) pathway regulates eIF4EBP1 through phosphorylation. In response to growth signals mTOR phosphorylates eIF4EBP1 causing the release of eIF4E and allowing mRNA translation to proceed. This interaction links eIF4EBP1 to the PI3K/AKT/mTOR signaling pathway which influences cell cycle progression and survival. Related proteins in this pathway include ribosomal protein S6 kinase (S6K1) which is also phosphorylated by mTOR to promote protein synthesis.

Deregulation of eIF4EBP1 has been implicated in cancer and neurological disorders. In cancer hyperactive mTOR signaling can lead to excessive phosphorylation of 4EBP1 decreasing its ability to inhibit eIF4E and enhancing translation of oncogenic proteins. In neurological disorders imbalanced eIF4EBP1 activity can disrupt synaptic plasticity and memory formation. Proteins such as p70S6K which are also part of the mTOR pathway share connections with eIF4EBP1 in these pathological conditions.
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일반 정보

기능

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex : hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

서열 유사성

Belongs to the eIF4E-binding protein family.

Post-translational modifications

Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF (PubMed:12588975, PubMed:12747827, PubMed:22578813, PubMed:24403073, PubMed:29236692, PubMed:7935836, PubMed:9465032). Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E (PubMed:12588975, PubMed:12747827, PubMed:22578813, PubMed:24403073, PubMed:29236692, PubMed:7935836, PubMed:9465032).. Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-37, Thr-46, Ser-65 and Thr-70) or associated with eIF4E.

Subcellular localisation

Nucleus

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제품 프로토콜

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타겟 정보

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex : hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
See full target information EIF4EBP1

대체 명칭 보기

Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, EIF4EBP1
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Product promise

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