Recombinant Human eNOS protein (His-DDDDK tag N-Terminus)
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Recombinant Human eNOS protein (His-DDDDK tag N-Terminus) is a Human Full Length protein, in the 2 to 1203 aa range, expressed in Baculovirus infected Sf9 cells, with >71%, suitable for SDS-PAGE.
대체 명칭 보기
Nitric oxide synthase 3, Constitutive NOS, EC-NOS, NOS type III, cNOS, NOSIII, Endothelial NOS, eNOS, NOS3
- WB
Supplier Data
Western blot - Recombinant Human eNOS protein (His-DDDDK tag N-Terminus) (AB198066)
Blocking/Dilution buffer : 5% NFDM/TBST. Exposure time : Lane 1 : 1 second; Lane 2 : 3 minutes.
All lanes:
Western blot - Anti-eNOS antibody [EPR19296] (<a href='/ko/products/primary-antibodies/enos-antibody-epr19296-ab199956'>ab199956</a>) at 1/1000 dilution
Lane 1:
Western blot - Recombinant Human eNOS protein (His-DDDDK tag N-Terminus) (ab198066) at 0.01 µg
Lane 2:
Western blot - Recombinant Human iNOS protein (His-DDDDK tag N-Terminus) (<a href='/ko/products/proteins-peptides/recombinant-human-inos-protein-ab135010'>ab135010</a>) at 0.05 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 133 kDa
Observed band size: 140 kDa
false
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human eNOS protein (His-DDDDK tag N-Terminus) (AB198066)
4-20% SDS-PAGE analysis of 1.6 μg ab198066 with Coomassie staining.
보완 제품
Reagents, Controls & Accessories
AB313995
Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)
- 0
- 0
Reactivity 정보
서열 정보
특성 및 보관 정보
제형
Purification 테크닉
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ENOS contributes significantly to the regulation of vascular tone and blood flow by promoting vasodilation. It does not function alone; instead it forms complexes with other proteins to exert its full effect. eNOS activity influences the process of angiogenesis inflammation modulation and platelet aggregation. Through its ability to produce nitric oxide eNOS acts as a signaling molecule helping maintain vascular homeostasis.
Pathways
ENOS is a critical player in the NO signaling pathway and interacts intricately with the PI3K/Akt pathway. Nitric oxide produced by eNOS has a role in signaling cascades that lead to vascular dilation and reduced blood pressure. The PI3K/Akt pathway regulates eNOS activity via phosphorylation enhancing NO production. Other proteins like caveolin-1 and calmodulin modulate eNOS impacting these pathways' outcomes.
일반 정보
기능
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed : 1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.. Isoform eNOS13C. Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
서열 유사성
Belongs to the NOS family.
Post-translational modifications
Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
Subcellular localisation
Cytoskeleton
타겟 정보
Product promise
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