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AB276396

Recombinant Human Factor IX/PTC protein (His tag)

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Recombinant Human Factor IX/PTC protein (His tag) is a Human Full Length protein, in the 1 to 461 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

대체 명칭 보기

Coagulation factor IX, Christmas factor, Plasma thromboplastin component, PTC, F9

1 이미지
SDS-PAGE - Recombinant Human Factor IX/PTC protein (His tag) (AB276396)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Factor IX/PTC protein (His tag) (AB276396)

SDS-PAGE analysis of ab276396

주요 정보

Purity

>95% SDS-PAGE

Endotoxin 레벨

< 1 EU/µg

발현 시스템

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.4 Constituents: PBS

storage-buffer

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보완 제품

Assay Kits

AB300307

Human Factor IX/PTC ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

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  • 1
Proteins & Peptides

AB316620

Recombinant Human CD46 Protein Standard (His tag)

Sandwich ELISA - Recombinant Human CD46 Protein Standard (His tag) (AB316620)

  • 0
  • 0
Proteins & Peptides

AB200264

Recombinant human Plasminogen protein (Active)

Bioactive

  • 0
  • 0
Proteins & Peptides

AB158410

Recombinant Human Factor XII protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Factor XII protein (GST tag N-Terminus) (AB158410)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MQRVNMIMAESPGLITICLLGYLLSAECTVFLDHENANKILNRPKRYNSGKLEEFVQGNLERECMEEKCSFEEAREVFENTERTTEFWKQYVDGDQCESNPCLNGGSCKDDINSYECWCPFGFEGKNCELDVTCNIKNGRCEQFCKNSADNKVVCSCTEGYRLAENQKSCEPAVPFPCGRVSVSQTSKLTRAETVFPDVDYVNSTEAETILDNITQSTQSFNDFTRVVGGEDAKPGQFPWQVVLNGKVDAFCGGSIVNEKWIVTAAHCVETGVKITVVAGEHNIEETEHTEQKRNVIRIIPHHNYNAAINKYNHDIALLELDEPLVLNSYVTPICIADKEYTNIFLKFGSGYVSGWGRVFHKGRSALVLQYLRVPLVDRATCLRSTKFTIYNNMFCAGFHEGGRDSCQGDSGGPHVTEVEGTSFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKTKLT","proteinLength":"Full Length","predictedMolecularWeight":"50 kDa","actualMolecularWeight":null,"aminoAcidEnd":461,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P00740","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Factor IX also known as Christmas factor or PTC is an important clotting protein in the coagulation cascade. It is a 415 amino acid protein with a mass of approximately 55 kDa. Factor IX is mainly expressed in the liver and then released into the bloodstream where it plays a significant role in blood clotting processes. The recombinant version of this protein referred to as factor IX recombinant is used therapeutically especially in patients who require coagulation support due to factor deficiencies.
Biological function summary

The function of factor IX involves its role in the amplification phase of the coagulation cascade. It participates as part of the intrinsic tenase complex which forms on negatively charged phospholipids in the presence of calcium ions. This complex significantly enhances the conversion of factor X to its active form factor Xa an important step in the clotting process. The activity of factor IX can be assessed using assays such as the factor IX activity assay and ELISA allowing for precise measurement of its function and quantity.

Pathways

Factor IX is integral within the intrinsic pathway of blood coagulation. It interacts with proteins such as factor VIII and factor X. When activated factor IXa forms a complex with the cofactor factor VIIIa on membrane surfaces intensifying the transformation of factor X to factor Xa which then catalyzes the conversion of prothrombin to thrombin. This series of reactions leads to fibrin formation important for stable clot production. Factor IXa also relates to the pathways influencing cellular signaling and hemostasis.

Factor IX deficiencies cause hemophilia B a genetic disorder that leads to improper blood clotting. This disorder is mainly characterized by spontaneous bleeding or severe bleeding after injury. Patients with hemophilia B usually have mutations in the factor IX gene on the X chromosome affecting the protein's function. Successful treatment often involves replacement therapy using factor IX concentrates or the recombinant form. The interaction with proteases such as factor VIIa highlights its importance in therapeutic strategies for bleeding disorders.
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일반 정보

기능

Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa (PubMed : 8295821, PubMed : 2592373, PubMed : 20121197, PubMed : 20121198, PubMed : 1730085, PubMed : 19846852, PubMed : 39880037).

서열 유사성

Belongs to the peptidase S1 family.

Post-translational modifications

Activated by factor XIa, which excises the activation peptide (PubMed:1730085, PubMed:9169594, PubMed:22961984). The propeptide can also be removed by snake venom protease (PubMed:20004170, PubMed:20080729). Activated by coagulation factor VIIa-tissue factor (F7-F3) complex in calcium-dependent manner (PubMed:271951).. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.. Predominantly O-glucosylated at Ser-99 by POGLUT1 in vitro.. Carboxylated by vitamin K-dependent GGCX to form gamma-carboxyglutamate; these residues are essential for the binding of calcium.

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제품 프로토콜

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타겟 정보

Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa (PubMed : 8295821, PubMed : 2592373, PubMed : 20121197, PubMed : 20121198, PubMed : 1730085, PubMed : 19846852, PubMed : 39880037).
See full target information Coagulation factor IX
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Product promise

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