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AB158403

Recombinant Human Factor VIII protein (GST tag N-Terminus)

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Recombinant Human Factor VIII protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 216 aa range, expressed in Wheat germ, suitable for ELISA, WB.

대체 명칭 보기

F8C, F8, Coagulation factor VIII, Antihemophilic factor, Procoagulant component, AHF

1 이미지
SDS-PAGE - Recombinant Human Factor VIII protein (GST tag N-Terminus) (AB158403)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Factor VIII protein (GST tag N-Terminus) (AB158403)

ab158403 on a 12.5% SDS-PAGE stained with Coomassie Blue.

주요 정보

발현 시스템

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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Primary Antibodies

AB61370

Anti-Factor VIII antibody

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Proteins & Peptides

AB276605

Recombinant Human SEZ6L2 protein (His tag)

SDS-PAGE - Recombinant Human SEZ6L2 protein (His tag) (AB276605)

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Proteins & Peptides

AB131757

Recombinant Human Factor H protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Factor H protein (GST tag N-Terminus) (AB131757)

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Proteins & Peptides

AB158401

Recombinant Human Factor VII protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Factor VII protein (GST tag N-Terminus) (AB158401)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MRIQDPGKVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGVTTQGVKSLLTSMYVKEFLISSSQDGHQWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY","proteinLength":"Full Length","predictedMolecularWeight":"51 kDa","actualMolecularWeight":null,"aminoAcidEnd":216,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P00451","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Proprietary technique
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Factor VIII also known as anti-hemophilic factor is a glycoprotein with a molecular mass of approximately 250 kDa. This protein circulates in the plasma mainly in the liver where hepatocytes produce it. Factor VIII remains inactive while circulating in the blood and exists within the factor VIII/von Willebrand factor complex. Upon activation it plays a significant role in the blood coagulation process.
Biological function summary

Factor VIII greatly contributes to hemostasis by serving as a cofactor for factor IXa forming the intrinsic tenase complex. This activation results in the conversion of factor X to factor Xa which is important for the common pathway of the coagulation cascade. By participating in the clotting process factor VIII ensures proper wound healing and prevention of blood loss.

Pathways

Factor VIII activity is pivotal within the coagulation cascade particularly the intrinsic and common pathways. Activation of the intrinsic pathway begins through interactions with factor IXa leading to efficient activation of factor X. This process closely relates factor VIII to proteins like factor IX and factor X ensuring proper clot formation and stabilization. Factor VIII products and assays are available to study and characterize its critical role in these pathways.

Factor VIII is closely associated with hemophilia A a genetic bleeding disorder resulting from deficiency or dysfunction of this protein. Patients with hemophilia A exhibit prolonged bleeding times due to impaired coagulation. The disorder links directly to von Willebrand factor as it protects factor VIII from degradation in circulation stabilizing its presence in the blood. Anti-factor VIII antibodies are important tools for diagnosing and studying factor VIII-related disorders.
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일반 정보

기능

Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.

서열 유사성

Belongs to the multicopper oxidase family.

Post-translational modifications

Sulfation on Tyr-1699 is essential for binding vWF.. Proteolytically cleaved by cathepsin CTSG to produce a partially activated form.

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제품 프로토콜

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타겟 정보

Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.
See full target information F8
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Mediterranean journal of hematology and infectious diseases 13:e2021041 PubMed34276910

2021

Hsa-miR-5581-3p and Hsa-miR-542-3p Target the Gene in Hemophilia A without Mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Feiying Meng
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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