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AB183258

Recombinant Human FDFT1 protein (denatured) (His tag N-Terminus)

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Recombinant Human FDFT1 protein (denatured) (His tag N-Terminus) is a Human Fragment protein, in the 1 to 283 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

대체 명칭 보기

Squalene synthase, SQS, SS, FPP:FPP farnesyltransferase, Farnesyl-diphosphate farnesyltransferase, Farnesyl-diphosphate farnesyltransferase 1, FDFT1

1 이미지
SDS-PAGE - Recombinant Human FDFT1 protein (denatured) (His tag N-Terminus) (AB183258)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human FDFT1 protein (denatured) (His tag N-Terminus) (AB183258)

15% SDS-PAGE analysis of ab183258 (3μg).

주요 정보

Purity

>85% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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보완 제품

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Primary Antibodies

AB195046

Anti-FDFT1 antibody [EPR16481]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FDFT1 antibody [EPR16481] (AB195046)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMEFVKCLGHPEEFYNLVRFRIGGKRKVMPKMDQDSLSSSLKTCYKYLNQTSRSFAAVIQALDGEMRNAVCIFYLVLRALDTLEDDMTISVEKKVPLLHNFHSFLYQPDWRFMESKEKDRQVLEDFPTISLEFRNLAEKYQTVIADICRRMGIGMAEFLDKHVTSEQEWDKYCHYVAGLVGIGLSRLFSASEFEDPLVGEDTERANSMGLFLQKTNIIRDYLEDQQGGREFWPQEVWSRYVKKLGDFAKPENIDLAVQCLNELITNALHHIPDVITYLSRLRNQ","proteinLength":"Fragment","predictedMolecularWeight":"35.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":283,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P37268","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FDFT1 also known as squalene synthase catalyzes the first committed step in cholesterol biosynthesis. This enzyme functions as a dimer and has a molecular weight of approximately 47 kDa. FDFT1 is widely expressed in the liver where cholesterol synthesis primarily occurs but you can also find it present in many tissues throughout the body. As a membrane-bound protein it anchors to the endoplasmic reticulum where it carries out its enzymatic role.
Biological function summary

FDFT1 facilitates the conversion of farnesyl diphosphate to squalene a critical precursor in sterol and cholesterol synthesis. This enzyme does not work in isolation but acts as a part of the larger sterol biosynthesis pathway. The activity of FDFT1 aligns with key metabolic demands particularly in tissues with high rates of cell division or hormone production making it essential for maintaining cellular sterol homeostasis.

Pathways

FDFT1 is an important enzyme in the mevalonate pathway and cholesterol biosynthesis pathway. This enzyme interacts closely with proteins such as HMG-CoA reductase and lanosterol synthase which are pivotal in these pathways. This relationship ensures the proper flow from acetyl-CoA to cholesterol and other isoprenoids critical for cell membrane integrity and signaling functions.

Disruptions in FDFT1 activity can lead to conditions like hypercholesterolemia and related cardiovascular diseases. Excess or deficiency in its function impacts cholesterol levels directly making it a target for cholesterol-lowering therapies. FDFT1's activity also connects to the proteins NPC1 and LDL receptor which play a role in cholesterol transport and uptake further linking it to pathways that contribute to atherosclerosis when dysregulated.
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일반 정보

기능

Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway.

서열 유사성

Belongs to the phytoene/squalene synthase family.

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제품 프로토콜

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타겟 정보

Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway.
See full target information FDFT1
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Product promise

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