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AB115708

Recombinant Human FGE protein (denatured) (His tag N-Terminus)

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Recombinant Human FGE protein (denatured) (His tag N-Terminus) is a Human Fragment protein, in the 91 to 374 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

대체 명칭 보기

PSEC0152, UNQ3037/PRO9852, SUMF1, Formylglycine-generating enzyme, FGE, C-alpha-formylglycine-generating enzyme 1, Sulfatase-modifying factor 1

1 이미지
SDS-PAGE - Recombinant Human FGE protein (denatured) (His tag N-Terminus) (AB115708)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human FGE protein (denatured) (His tag N-Terminus) (AB115708)

15% SDS-PAGE showing ab115708 at approximately 34.1kDa (3μg).

주요 정보

Purity

>85% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 12.01% Urea, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB234617

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB234617)

  • 0
  • 0
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Primary Antibodies

AB187516

Anti-GALNS/Chondroitinase antibody

Western blot - Anti-GALNS/Chondroitinase antibody (AB187516)

  • 0
  • 0
Secondary Antibodies

AB99817

Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP)

ELISA - Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP) (AB99817)

  • 0
  • 0
Secondary Antibodies

AB6734

Rabbit Anti-Rat IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (AB6734)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

This product was previously labelled as SUMF1
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMVPIPAGVFTMGTDDPQIKQDGEAPARRVTIDAFYMDAYEVSNTEFEKFVNSTGYLTEAEKFGDSFVFEGMLSEQVKTNIQQAVAAAPWWLPVKGANWRHPEGPDSTILHRPDHPVLHVSWNDAVAYCTWAGKRLPTEAEWEYSCRGGLHNRLFPWGNKLQPKGQHYANIWQGEFPVTNTGEDGFQGTAPVDAFPPNGYGLYNIVGNAWEWTSDWWTVHHSVEETLNPKGPPSGKDRVKKGGSYMCHRSYCYRYRCAARSQNTPDSSASNLGFRCAADRLPTMD","proteinLength":"Fragment","predictedMolecularWeight":"34.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":374,"aminoAcidStart":91,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8NBK3","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The FGE or Formylglycine-generating enzyme also known as SUMF1 plays a mechanical role by catalyzing the post-translational activation of sulfatases. This enzyme exists within the endoplasmic reticulum and possesses a molecular weight of approximately 35 kDa. FGE expression occurs broadly across various tissues reflecting its necessity for cellular function. Its action involves converting a specific cysteine residue to formylglycine in sulfatase enzymes essential for their functional activity.
Biological function summary

Formylglycine-generating enzyme contributes significantly to the functionality of sulfatases which carry out essential steps in the degradation of sulfate-containing molecules. This conversion is not a part of a larger complex but enables critical activities through sulfatases. Sulfatases require formylglycine for activity highlighting FGE's role in regulating their presence and function within the cell.

Pathways

Formylglycine-generating enzyme integrates within metabolic pathways involved in glycosaminoglycan degradation. Through this action it connects to proteins like arylsulfatases essential in hydrolyzing sulfate groups from complex carbohydrates. This hydrolysis occurs within the lysosomal degradation pathway where FGE-activated sulfatases function bridging metabolic processes important for cellular maintenance.

Mutations or deficiencies in formylglycine-generating enzyme lead to multiple sulfatase deficiency (MSD) a disorder affecting lysosomal function and causing progressive neurological damage. In this context alterations in this enzyme impact related proteins such as ARSA and other sulfatases disrupting normal catabolic processes and leading to the accumulation of sulfated compounds. Additionally FGE deficiencies connect to skeletal dysplasias illustrating the broad physiological impact this enzyme holds when its function is compromised.
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일반 정보

기능

Oxidase that catalyzes the conversion of cysteine to 3-oxoalanine on target proteins, using molecular oxygen and an unidentified reducing agent (PubMed : 12757706, PubMed : 15657036, PubMed : 15907468, PubMed : 16368756, PubMed : 21224894, PubMed : 25931126). 3-oxoalanine modification, which is also named formylglycine (fGly), occurs in the maturation of arylsulfatases and some alkaline phosphatases that use the hydrated form of 3-oxoalanine as a catalytic nucleophile (PubMed : 12757706, PubMed : 15657036, PubMed : 15907468, PubMed : 16368756, PubMed : 25931126). Known substrates include GALNS, ARSA, STS and ARSE (PubMed : 12757706, PubMed : 15657036, PubMed : 15907468).

서열 유사성

Belongs to the sulfatase-modifying factor family.

Post-translational modifications

N-glycosylated. Contains high-mannose-type oligosaccharides.

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제품 프로토콜

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타겟 정보

Oxidase that catalyzes the conversion of cysteine to 3-oxoalanine on target proteins, using molecular oxygen and an unidentified reducing agent (PubMed : 12757706, PubMed : 15657036, PubMed : 15907468, PubMed : 16368756, PubMed : 21224894, PubMed : 25931126). 3-oxoalanine modification, which is also named formylglycine (fGly), occurs in the maturation of arylsulfatases and some alkaline phosphatases that use the hydrated form of 3-oxoalanine as a catalytic nucleophile (PubMed : 12757706, PubMed : 15657036, PubMed : 15907468, PubMed : 16368756, PubMed : 25931126). Known substrates include GALNS, ARSA, STS and ARSE (PubMed : 12757706, PubMed : 15657036, PubMed : 15907468).
See full target information SUMF1
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Product promise

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