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AB256004

Recombinant human FGF9/GAF protein (Animal Free)

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Recombinant human FGF9/GAF protein (Animal Free) is a Human Fragment protein, in the 3 to 208 aa range, expressed in Escherichia coli, with >95%, <1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

대체 명칭 보기

Fibroblast growth factor 9, FGF-9, Glia-activating factor, Heparin-binding growth factor 9, GAF, HBGF-9, FGF9

2 이미지
Functional Studies - Recombinant human FGF9/GAF protein (Animal Free) (AB256004)
  • FuncS

Supplier Data

Functional Studies - Recombinant human FGF9/GAF protein (Animal Free) (AB256004)

ab256004 induced proliferation of NR6R-3T3 cells.

SDS-PAGE - Recombinant human FGF9/GAF protein (Animal Free) (AB256004)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human FGF9/GAF protein (Animal Free) (AB256004)

SDS-PAGE analysis of ab256004 (1 μg) under non-reducing (Lane 1) and reducing (Lane 2) conditions.

4-20% Tris-Glycine gel. Coomassie Blue staining.

주요 정보

Purity

>95% SDS-PAGE

Endotoxin 레벨

<1 EU/µg

발현 시스템

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

NR6R-3T3 cell proliferation: ED50 ≤ 2 ng/ml (≥ 5.0 x 105 units/mg).

Accession

Animal free

Yes

Carrier free

Yes

Species

Human

Reconstitution

Reconstitute at 0.1 mg/mL in water

보관 버퍼

Constituents: 0.71% Sodium sulphate, 0.16% Sodium phosphate, 0.15% Sodium chloride

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

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  • 0
Primary Antibodies

AB89901

Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (AB89901)

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  • 43
Primary Antibodies

AB115821

Anti-FGF9/GAF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF9/GAF antibody (AB115821)

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Biochemicals

AB142044

Reduced Glutathione (GSH), Endogenous antioxidant

Chemical Structure - Reduced Glutathione (GSH), Endogenous antioxidant (AB142044)

  • 0
  • 0
Assay Kits

AB183360

Human FAS ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human FAS ELISA Kit (AB183360)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MPLGEVGNYFGVQDAVPFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS","proteinLength":"Fragment","predictedMolecularWeight":"23.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":208,"aminoAcidStart":3,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P31371","tags":[]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FGF9 also known as GAF is a member of the fibroblast growth factor family. The FGF9 protein has a molecular mass of approximately 23 kDa. It is commonly expressed in the brain kidney and lungs during fetal development. The FGF9 protein functions as a signaling molecule influencing cell proliferation and differentiation which are essential for proper tissue formation and organogenesis.
Biological function summary

FGF9 contributes to important processes in the development and maintenance of several tissues. It is not known to be part of a larger complex. FGF9 regulates cellular activities such as proliferation and differentiation through its interaction with specific receptors on the cell surface. Its role is significant in skeletal and nervous system development where it ensures proper maturation and function of these systems.

Pathways

The FGF9 protein participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are important for cell survival proliferation and differentiation. FGF9 interacts closely with other fibroblast growth factors such as FGF2 to ensure effective signaling cascades. These interactions affect various cellular responses and contribute to the normal development of multiple organ systems.

Alterations in FGF9 expression can be linked to conditions such as lung fibrosis and cancer. Aberrant FGF9 signaling can contribute to uncontrolled cell proliferation and fibrosis formation. The GAF protein's dysregulation may involve interactions with receptor proteins including FGFR2 in these pathological processes. Understanding FGF9's role in these diseases helps in developing targeted therapeutic strategies.
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일반 정보

기능

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.

서열 유사성

Belongs to the heparin-binding growth factors family.

Post-translational modifications

Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.. N-glycosylated.

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제품 프로토콜

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타겟 정보

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
See full target information Fibroblast growth factor 9
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 25: PubMed38732014

2024

Fetal Kidney Grafts and Organoids from Microminiature Pigs: Establishing a Protocol for Production and Long-Term Cryopreservation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuka Inage,Koki Fujimori,Masaki Takasu,Kenji Matsui,Yoshitaka Kinoshita,Keita Morimoto,Nagisa Koda,Shutaro Yamamoto,Kentaro Shimada,Takashi Yokoo,Eiji Kobayashi
제품이 사용된 논문 모두 보기
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Product promise

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