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AB131684

Recombinant Human FTSJ2 protein (His tag N-Terminus)

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Recombinant Human FTSJ2 protein (His tag N-Terminus) is a Human Fragment protein, in the 51 to 246 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

FJH1, FTSJ2, MRM2, 16S rRNA (uridine(1369)-2'-O)-methyltransferase, 16S rRNA [Um1369] 2'-O-methyltransferase, Protein ftsJ homolog 2

1 이미지
SDS-PAGE - Recombinant Human FTSJ2 protein (His tag N-Terminus) (AB131684)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human FTSJ2 protein (His tag N-Terminus) (AB131684)

15% SDS-PAGE analysis of 3 µg ab131684.

주요 정보

Purity

>95% SDS-PAGE

ab131684 was purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Primary Antibodies

AB60068

Anti-FTSJ2 antibody [2372C6a]

Western blot - Anti-FTSJ2 antibody [2372C6a] (AB60068)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSSYRCRSAFKLLEVNERHQILRPGLRVLDCGAAPGAWSQVAVQKVNAAGTDPSSPVGFVLGVDLLHIFPLEGATFLCPADVTDPRTSQRILEVLPGRRADVILSDMAPNATGFRDLDHDRLISLCLTLLSVTPDILQPGGTFLCKTWAGSQSRRLQRRLTEEFQNVRIIKPEASRKESSEVYFLATQYHGRKGTVKQ","proteinLength":"Fragment","predictedMolecularWeight":"24.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":246,"aminoAcidStart":51,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UI43","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FTSJ2 also known as hPrmA or methyltransferase-like protein 1 is an enzyme with a mass of approximately 83 kDa. It functions as a human 2'-O-methyltransferase and takes part in ribosomal RNA modification by methylating the ribose moiety in RNA molecules. You can find FTSJ2 widely expressed in various human tissues with notable expression in the liver and brain. The enzyme localizes mainly in the nucleolus a site central to ribosomal RNA biosynthesis and modification activities.
Biological function summary

The enzymatic activities of FTSJ2 contribute to ribosome assembly and stability by modifying specific rRNA components. These modifications are essential for the accurate assembly of ribosomal subunits and the proper function of the translation machinery within the cell. FTSJ2 does not function in isolation; it often operates as part of multiprotein complexes which assist in guiding and regulating its methyltransferase activity.

Pathways

Methylation activity by FTSJ2 plays a critical role in the RNA processing pathway and cellular translation machinery. It directly affects the biogenesis of rRNA ensuring the efficient assembly of ribosomal units required for protein synthesis. FTSJ2 interacts with other proteins such as NOP10 and NOP56 which are potentials linking it to the broader ribonucleoprotein complex within cells.

Alterations in FTSJ2 activity and expression have connections to several human conditions notably cancer and neurological disorders. Aberrations in the rRNA modification process could lead to disruptions in protein synthesis and uncontrolled cell proliferation which are often observed in cancerous cells. In the context of neurological disorders FTSJ2's function links to proteins like FBL which play key roles in neural cell RNA processing. Changes in their function could potentially contribute to neurodegenerative conditions.
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일반 정보

기능

S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methyluridine at position 1369 (Um1369) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a universally conserved modification in the peptidyl transferase domain of the mtLSU rRNA (PubMed : 25009282, PubMed : 25074936, PubMed : 35177605). This activity may require prior 2'-O-methylguanosine modification at position 1370 (Gm1370) by MRM3 (PubMed : 35177605). Essential for late-stage assembly of mtLSU required for efficient translation of mitochondrial DNA encoded proteins; methyltransferase activity is not required for this function (PubMed : 35177605). Essential for mitochondrial respiratory function (PubMed : 35177605).

서열 유사성

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.

Subcellular localisation

Mitochondrion

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제품 프로토콜

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타겟 정보

S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methyluridine at position 1369 (Um1369) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a universally conserved modification in the peptidyl transferase domain of the mtLSU rRNA (PubMed : 25009282, PubMed : 25074936, PubMed : 35177605). This activity may require prior 2'-O-methylguanosine modification at position 1370 (Gm1370) by MRM3 (PubMed : 35177605). Essential for late-stage assembly of mtLSU required for efficient translation of mitochondrial DNA encoded proteins; methyltransferase activity is not required for this function (PubMed : 35177605). Essential for mitochondrial respiratory function (PubMed : 35177605).
See full target information MRM2
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Product promise

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