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AB323148

Recombinant Human GAB1 Protein Standard (His tag)

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Recombinant Human GAB1 Protein Standard (His tag) is a Human Fragment protein, expressed in Escherichia coli, with >80%, suitable for sELISA, SDS-PAGE.
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Sandwich ELISA - Recombinant Human GAB1 Protein Standard (His tag) (AB323148)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human GAB1 Protein Standard (His tag) (AB323148)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human GAB1 Protein Standard (His tag) (AB323148)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human GAB1 Protein Standard (His tag) (AB323148)

SDS-PAGE analysis of ab323148 under reducing conditions for 2ug protein.

주요 정보

Purity

>80% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

Q13480-1

Animal free

Yes

Carrier free

No

Species

Human

보관 버퍼

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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보완 제품

Proteins & Peptides

AB85917

Recombinant Human GRB2 protein (His tag N-Terminus)

Western blot - Recombinant Human GRB2 protein (His tag N-Terminus) (AB85917)

  • 0
  • 0
Proteins & Peptides

AB233594

Recombinant Human SOS1 protein (His tag C-Terminus)

SDS-PAGE - Recombinant Human SOS1 protein (His tag C-Terminus) (AB233594)

  • 0
  • 0
Primary Antibodies

AB133486

Anti-GAB1 antibody [EPR375]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-GAB1 antibody [EPR375] (AB133486)

  • 0
  • 0
Biochemicals

AB145861

L-Mimosine, non-protein amino acid

Chemical Structure - L-Mimosine, non-protein amino acid (AB145861)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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서열 정보

[{"linker":null,"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"42.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q13480","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification His Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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일반 정보

기능

Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway (PubMed : 29408807).

서열 유사성

Belongs to the GAB family.

Post-translational modifications

Phosphorylated in response to FGFR1 activation. Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.

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제품 프로토콜

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타겟 정보

Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway (PubMed : 29408807).
See full target information GAB1

대체 명칭 보기

GRB2-associated-binding protein 1, GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1, GAB1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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