AB206646

Recombinant human GAD65 protein

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Recombinant human GAD65 protein is a Human Full Length protein, in the 1 to 585 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, < 5 EU/mL endotoxin level, suitable for SDS-PAGE, WB, FuncS.

대체 명칭 보기

GAD65, GAD2, Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform, GAD-65

1 이미지

SDS-PAGE - Recombinant human GAD65 protein (AB206646)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human GAD65 protein (AB206646)

SDS Page Gel analysis comparing different protein molecular weights, lanes 1 & 2 contain rhGAD65 ab206647 at concentrations of 1 μL & 0.1 μL respectively. This protein was compared to BSA (lane 3 - 1 μg & lane 4 - 10 μg) and T-cell GAD65 (ab206646) (lane 5 - 1 μL & lane 6 - 0.1 μL). The bands were run on a NuPAGE 7% Tris-Acetate Gel.

주요 정보

Purity

>95% SDS-PAGE

Purified by SDS-PAGE and RP-HPLC.

Endotoxin 레벨

< 5 EU/mL

발현 시스템

Baculovirus infected Sf9 cells

Applications

FuncS, SDS-PAGE, WB

applications

Biologically active

Yes

Biological activity

Active

Accession

Animal free

Carrier free

Species

Human

보관 버퍼

Constituents: Human serum albumin, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Recombinant human GAD65 homogeneity confirmed by SDS-PAGE.Also by IEF and Size Exclusion Chromatography." }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Recombinant human GAD65 homogeneity confirmed by WB." }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"T cell stimulatory activity of T-cell GAD65 confirmed in in vitro assays." } } }

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서열 정보

[{"linker":null,"sequence":"MASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYGDAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL","proteinLength":"Full Length","predictedMolecularWeight":"65 kDa","actualMolecularWeight":null,"aminoAcidEnd":585,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q05329","tags":[]}]

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특성 및 보관 정보

제형

Liquid

배송 시 보관 조건

Dry Ice

적절한 단기 보관 조건

-80°C

적절한 장기 보관 조건

-80°C

보관 정보

Avoid freeze / thaw cycle

True

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일반 정보

기능

Catalyzes the production of GABA.

서열 유사성

Belongs to the group II decarboxylase family.

Post-translational modifications

Phosphorylated; which does not affect kinetic parameters or subcellular location.. Palmitoylated; which is required for presynaptic clustering.

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the production of GABA.

See full target information GAD2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biosensors 14: PubMed38785729

2024

Detection of Glutamate Decarboxylase Antibodies and Simultaneous Multi-Molecular Translocation Exploration by Glass Nanopores.

Applications

Unspecified application

Species

Unspecified reactive species

Chongxin Tao,Yun Bai,Jiang Chen,Jing Lu,Yan Bi,Jian Li

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant human GAD65 protein

SDS-PAGE - Recombinant human GAD65 protein (AB206646)

주요 정보

Purity

Endotoxin 레벨

발현 시스템

Tags

Applications

Biologically active

Biological activity

Accession

Animal free

Carrier free

Species

보관 버퍼

보완 제품

Reagents, Controls & Accessories

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity 정보

서열 정보

특성 및 보관 정보

제형

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

일반 정보

기능

서열 유사성

Post-translational modifications

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise