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AB99294

Recombinant Human Galectin 8/Gal-8 protein (His tag N-Terminus)

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Recombinant Human Galectin 8/Gal-8 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 317 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec, WB.
2 이미지
Western blot - Recombinant Human Galectin 8/Gal-8 protein (His tag N-Terminus) (AB99294)
  • WB

Unknown

Western blot - Recombinant Human Galectin 8/Gal-8 protein (His tag N-Terminus) (AB99294)

All lanes:

Western blot - Anti-Galectin 8/Gal-8 antibody [EPR4857] (<a href='/ko/products/primary-antibodies/galectin-8-gal-8-antibody-epr4857-ab109519'>ab109519</a>) at 1/5000 dilution

All lanes:

Western blot - Recombinant Human Galectin 8/Gal-8 protein (His tag N-Terminus) (ab99294) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 36 kDa

true

Exposure time: 90s

SDS-PAGE - Recombinant Human Galectin 8/Gal-8 protein (His tag N-Terminus) (AB99294)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Galectin 8/Gal-8 protein (His tag N-Terminus) (AB99294)

15% SDS-PAGE showing ab99294 (3μg).

주요 정보

Purity

>90% SDS-PAGE

ab99294 is purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE, WB

applications

Biologically active

No

Accession

O00214-1

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Proteins & Peptides

AB99239

Recombinant Human Galectin 2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Galectin 2 protein (His tag N-Terminus) (AB99239)

  • 0
  • 0
Proteins & Peptides

AB316415

Recombinant Mouse Galectin 3 Protein Standard (His tag)

Sandwich ELISA - Recombinant Mouse Galectin 3 Protein Standard (His tag) (AB316415)

  • 0
  • 0
Proteins & Peptides

AB283440

Recombinant Human Galectin 1 protein

SDS-PAGE - Recombinant Human Galectin 1 protein (AB283440)

  • 0
  • 0
Proteins & Peptides

AB107951

Recombinant Human Galectin 10 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Galectin 10 protein (His tag N-Terminus) (AB107951)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab99294 can be used as a WB positive control in conjunction with <a href='/ko/products/primary-antibodies/galectin-8-gal-8-antibody-epr4857-ab109519'>ab109519</a>.</p>" } } }
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제품 세부 정보

Previously labelled as Galectin 8.
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMMLSLNNLQNIIYNPVIPFVGTIPDQLDPGTLIVIRGHVPSDADRFQVDLQNGSSMKPRADVAFHFNPRFKRAGCIVCNTLINEKWGREEITYDTPFKREKSFEIVIMVLKDKFQVAVNGKHTLLYGHRIGPEKIDTLGIYGKVNIHSIGFSFSSDLQSTQASSLELTEISRENVPKSGTPQLRLPFAARLNTPMGPGRTVVVKGEVNANAKSFNVDLLAGKSKDIALHLNPRLNIKAFVRNSFLQESWGEEERNITSFPFSPGMYFEMIIYCDVREFKVAVNGVHSLEYKHRFKELSSIDTLEINGDIHLLEVRSW","proteinLength":"Full Length","predictedMolecularWeight":"37.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":317,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O00214","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Galectin 8 also known as Gal-8 or GAL is a member of the galectin protein family which binds β-galactosides. The protein has another name anti-gal and possesses a molecular mass of approximately 36 kDa. It exhibits broad expression in various tissues including the immune system bones and gastrointestinal tract. Galectin-8 functions mechanically by binding to glycoproteins and glycolipids which facilitates cell adhesion and regulation processes.
Biological function summary

Galectin-8 impacts numerous cellular functions through its involvement in immunomodulation and cell migration. Gal-8 promotes adhesion between cells and the extracellular matrix. It is not a part of a larger protein complex but interacts with multiple cell surface receptors guiding them toward or away from specific locations. Galectins including Galectin-8 influence immune cell responses by managing the apoptosis of T-cells and modifying cytokine production.

Pathways

Galectin-8 engages in signaling pathways related to cellular adhesion and immune system modulation. It participates in the integrin-mediated cell adhesion pathway and contributes to the receptor signaling pathway associated with cytokines. Within these pathways Galectin-8 interacts with other proteins such as integrins and T-cell receptors influencing their activation internalization and cell surface retention.

Galectin-8 plays roles in cancer progression and inflammatory conditions. It is connected to cancer as it regulates angiogenesis cell invasion and metastasis. Galectin-8 interacts with fibronectin and vascular endothelial growth factor (VEGF) within the context of cancer. In inflammatory diseases Gal-8 influences immune cell migration and inflammation through its action on cytokine receptors contributing to conditions like rheumatoid arthritis.
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일반 정보

기능

Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy (PubMed : 22246324, PubMed : 28077878). Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture (PubMed : 22246324, PubMed : 28077878). Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52 (PubMed : 22246324, PubMed : 28077878). Required to restrict infection of bacterial invasion such as S.typhimurium (PubMed : 22246324). Also required to restrict infection of Picornaviridae viruses (PubMed : 28077878). Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans (PubMed : 21288902).

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제품 프로토콜

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타겟 정보

Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy (PubMed : 22246324, PubMed : 28077878). Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture (PubMed : 22246324, PubMed : 28077878). Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52 (PubMed : 22246324, PubMed : 28077878). Required to restrict infection of bacterial invasion such as S.typhimurium (PubMed : 22246324). Also required to restrict infection of Picornaviridae viruses (PubMed : 28077878). Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans (PubMed : 21288902).
See full target information LGALS8

대체 명칭 보기

Galectin-8, Gal-8, Po66 carbohydrate-binding protein, Prostate carcinoma tumor antigen 1, Po66-CBP, PCTA-1, LGALS8
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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