AB114149

Recombinant Human GFAP protein (GST tag N-Terminus)

Recombinant Human GFAP 단백질 (GST tag N-Terminus)

Be the first to review this product! 리뷰 제출

(6 제품이 사용된 논문 )

Recombinant Human GFAP protein is a Human Full Length protein, in the 1 to 432 aa range, expressed in Wheat germ and suitable for SDS-PAGE, western blot and ELISA. The predicted molecular weight of ab114149 protein is 76 kDa.

- Save time and ensure accurate results - use our recombinant Glial fibrillary acidic protein (GFAP) as a control

2 이미지

Unknown

Western blot - Recombinant Human GFAP protein (GST tag N-Terminus) (AB114149)

All lanes:

Anti-GFAP antibody - Astrocyte Marker (<a href='/ko/products/unavailable/gfap-antibody-astrocyte-marker-ab48050'>ab48050</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human GFAP protein (GST tag N-Terminus) (ab114149) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 10s

SDS-PAGE - Recombinant Human GFAP protein (GST tag N-Terminus) (AB114149)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GFAP protein (GST tag N-Terminus) (AB114149)

12.5% SDS-PAGE analysis of ab114149, stained with Coomassie Blue.

주요 정보

발현 시스템

Wheat germ

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

Accession

P14136-1

Animal free

Carrier free

Species

Human

보관 버퍼

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab114149 can be used as a WB positive control in conjunction with <a href='/ko/products/unavailable/gfap-antibody-astrocyte-marker-ab48050'>ab48050</a>." } } }

style

left-column

제품 세부 정보

Ensure the validity of your result using our recombinant human GFAP protein ab114149 as a positive control in western blot and SDS-PAGE.

Analyze your GFAP ELISA data using the ab114149 protein to generate and plot a standard curve.

Check out our protein gel staining guide for SDS-PAGE here

Check out of western blot protocol for more information here

Check out our ELISA protocol for more information here.

style

left-column

서열 정보

[{"linker":null,"sequence":"MERRRITSAARRSYVSSGEMMVGGLAPGRRLGPGTRLSLARMPPPLPTRVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM","proteinLength":"Full Length","predictedMolecularWeight":"76 kDa","actualMolecularWeight":null,"aminoAcidEnd":432,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P14136","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Proprietary technique

배송 시 보관 조건

Dry Ice

적절한 단기 보관 조건

-80°C

적절한 장기 보관 조건

-80°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

False

style

left-column

style

left-column

제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

See full target information GFAP

대체 명칭 보기

Glial fibrillary acidic protein, GFAP

style

left-column

제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록and refine your search

Medicine 102:e32885 PubMed36820591

2023

The neuroprotective effect of electro-acupuncture on cognitive recovery for patients with mild traumatic brain injury: A randomized controlled clinical trial.

Applications

Unspecified application

Species

Unspecified reactive species

Haokun Jia,Yonghan Chen,Yi Wang,Linwei Jia,Yaohui Tian,Hao Jiang

Lupus science & medicine 9: PubMed35705307

2022

Role of autoantibodies and blood-brain barrier leakage in cognitive impairment in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

John G Hanly,Alexandra Legge,Lyna Kamintsky,Alon Friedman,Javeria A Hashmi,Steven D Beyea,John Fisk,Antonina Omisade,Cynthia Calkin,Tim Bardouille,Chris Bowen,Kara Matheson,Marvin J Fritzler

Journal of neural transmission (Vienna, Austria : 1996) 129:545-555 PubMed35364741

2022

Plasma autoantibodies to glial fibrillary acidic protein (GFAP) react with brain areas according to Braak staging of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Gschmack,Camelia-Maria Monoranu,Hecham Marouf,Sarah Meyer,Lena Lessel,Raja Idris,Daniela Berg,Walter Maetzler,Frank Steigerwald,Jens Volkmann,Manfred Gerlach,Peter Riederer,Eleni Koutsilieri,Carsten Scheller

Nanoscale advances 4:1770-1778 PubMed35434521

2022

Carbon dot-based fluorescent antibody nanoprobes as brain tumour glioblastoma diagnostics.

Applications

Unspecified application

Species

Unspecified reactive species

Mattia Ghirardello,Radhe Shyam,Xia Liu,Teodoro Garcia-Millan,Imke Sittel,Javier Ramos-Soriano,Kathreena M Kurian,M Carmen Galan

Neuropsychiatric disease and treatment 16:2633-2647 PubMed33177827

2020

Transcription Factor E2F1 Aggravates Neurological Injury in Ischemic Stroke via microRNA-122-Targeted Sprouty2.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxia Wu,Zhiqiang Gao,Jiang Zhang

Biosensors & bioelectronics 75:420-6 PubMed26356762

2015

Microwave-accelerated bioassay technique for rapid and quantitative detection of biological and environmental samples.

Applications

Unspecified application

Species

Unspecified reactive species

Muzaffer Mohammed,Maleeha F Syed,Kadir Aslan

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant Human GFAP protein (GST tag N-Terminus)

Recombinant Human GFAP 단백질 (GST tag N-Terminus)

Western blot - Recombinant Human GFAP protein (GST tag N-Terminus) (AB114149)

SDS-PAGE - Recombinant Human GFAP protein (GST tag N-Terminus) (AB114149)

주요 정보

발현 시스템

Tags

Applications

Biologically active

Accession

Animal free

Carrier free

Species

보관 버퍼

보완 제품

Reagents, Controls & Accessories

Assay Kits

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

서열 정보

특성 및 보관 정보

제형

Purification 테크닉

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise