AB89233

Recombinant Human Ghrelin protein (His tag N-Terminus)

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Recombinant Human Ghrelin protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB.

2 이미지

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Western blot - Recombinant Human Ghrelin protein (His tag N-Terminus) (AB89233)

All lanes:

Anti-Ghrelin antibody (<a href='/ko/products/unavailable/ghrelin-antibody-ab57222'>ab57222</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Ghrelin protein (His tag N-Terminus) (ab89233) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

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Exposure time: 2min

SDS-PAGE - Recombinant Human Ghrelin protein (His tag N-Terminus) (AB89233)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Ghrelin protein (His tag N-Terminus) (AB89233)

15% SDS-PAGE showing ab89233 (3μg).
Note : Real molecular weight on SDS-PAGE is shifted up compared to predicted molecular weight.

주요 정보

Purity

>90% SDS-PAGE

ab89233 is purified using conventional chromatography techniques.Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method).

발현 시스템

Escherichia coli

Applications

SDS-PAGE, WB

applications

Biologically active

Accession

Q9UBU3-1

Animal free

Carrier free

Species

Human

보관 버퍼

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.32% Tris HCl, 0.002% PMSF

storage-buffer

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab89233 can be used as a WB positive control in conjunction with <a href='/ko/products/unavailable/ghrelin-antibody-ab57222'>ab57222</a>.</p>" } } }

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서열 정보

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9UBU3","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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특성 및 보관 정보

제형

Liquid

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

False

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ghrelin also known as the "hunger hormone" or GH secretagogue is a peptide hormone with a mass of 28 amino acids. Ghrelin is mainly expressed in the stomach particularly in the enteroendocrine cells but it can also be found in small amounts in the lungs pancreas and hypothalamus. The hormone increases food intake and promotes fat storage by signaling the brain to stimulate hunger. Its secretion occurs in response to fasting and is suppressed by food intake reflecting its role in energy balance.

Biological function summary

Ghrelin influences a variety of physiological processes beyond hunger regulation. It plays a role in modulating the release of growth hormone by acting on the growth hormone secretagogue receptor (GHS-R). Ghrelin also affects gastric motility and acid secretion due to its expression in the gastrointestinal tract. Though not part of a larger complex its interaction with GHS-R strongly influences its biological functions. Ghrelin may also impact insulin secretion and sensitivity linking it to glucose metabolism processes.

Pathways

Ghrelin interacts with several critical biological networks. Ghrelin is involved in the neuroendocrine pathway that connects the gut to the brain and contributes significantly to energy homeostasis. Within this pathway it influences proteins like leptin which exerts opposite effects by suppressing hunger. Additionally ghrelin functions in the hedonic control of eating which relates to the rewarding aspects of food intake controlled by pathways involving dopamine.

Ghrelin holds relevance to obesity and cachexia a form of extreme weight loss often seen in chronic illness. Ghrelin promotes adiposity by stimulating appetite which can contribute to obesity. Conversely in cachexia the decrease in ghrelin activity is thought to exacerbate weight loss. Its role in energy balance associates ghrelin with the protein leptin which counterbalances its effects and connections to metabolic disorders. Understanding ghrelin's functions and pathways provides insight into therapeutic strategies for these conditions.

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일반 정보

기능

Ghrelin-27. Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) (PubMed : 10604470). Induces the release of growth hormone from the pituitary (PubMed : 10604470). Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.. Ghrelin-28. Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) (PubMed : 10604470). Induces the release of growth hormone from the pituitary (PubMed : 10604470). Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.. Obestatin. May be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).

서열 유사성

Belongs to the motilin family.

Post-translational modifications

O-octanoylated by GOAT/MBOAT4 (PubMed:18443287). O-octanoylation or O-decanoylation is essential for ghrelin activity (PubMed:10604470). The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26 (PubMed:10604470). A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid (PubMed:10604470). Also O-acetylated and O-butyrylated on Ser-26 to minor levels (PubMed:18443287).. Amidation of Leu-98 is essential for obestatin activity.

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information GHRL

대체 명칭 보기

MTLRP, UNQ524/PRO1066, GHRL, Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of biomolecular screening 18:1035-42 PubMed23704134

2013

Establishment of a gastric cell-based assay system for exploring inhibitors of octanoylated ghrelin production.

Applications

Unspecified application

Species

Unspecified reactive species

Shigeru Oiso,Miyuki Nobe,Yuhei Yamaguchi,Shigeru Umemoto,Kazuo Nakamura,Hiroko Kariyazono

제품이 사용된 논문 모두 보기

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Product promise

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Recombinant Human Ghrelin protein (His tag N-Terminus)

Western blot - Recombinant Human Ghrelin protein (His tag N-Terminus) (AB89233)

SDS-PAGE - Recombinant Human Ghrelin protein (His tag N-Terminus) (AB89233)

주요 정보

Purity

발현 시스템

Tags

Applications

Biologically active

Accession

Animal free

Carrier free

Species

보관 버퍼

보완 제품

Reagents, Controls & Accessories

Proteins & Peptides

Proteins & Peptides

Proteins & Peptides

Proteins & Peptides

Reactivity 정보

서열 정보

특성 및 보관 정보

제형

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

일반 정보

기능

서열 유사성

Post-translational modifications

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise