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AB271528

Recombinant human GITR protein (Active) (Biotin) (Avi tag C-Terminus + Fc tag C-Terminus)

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Recombinant human GITR protein (Active) (Biotin) (Avi tag C-Terminus + Fc tag C-Terminus) is a Human Fragment protein, in the 26 to 161 aa range, expressed in HEK 293 cells, with >90%, suitable for FuncS, SDS-PAGE.

대체 명칭 보기

CD357, AITR, GITR, UNQ319/PRO364, TNFRSF18, Tumor necrosis factor receptor superfamily member 18, Activation-inducible TNFR family receptor, Glucocorticoid-induced TNFR-related protein

2 이미지
Functional Studies - Recombinant human GITR protein (Active) (Biotin) (Avi tag C-Terminus + Fc tag C-Terminus) (AB271528)
  • FuncS

Unknown

Functional Studies - Recombinant human GITR protein (Active) (Biotin) (Avi tag C-Terminus + Fc tag C-Terminus) (AB271528)

GITRL : GITR-biotin binding.

SDS-PAGE - Recombinant human GITR protein (Active) (Biotin) (Avi tag C-Terminus + Fc tag C-Terminus) (AB271528)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human GITR protein (Active) (Biotin) (Avi tag C-Terminus + Fc tag C-Terminus) (AB271528)

SDS-PAGE analysis of 3 μg ab271528.

This protein runs at a higher molecular weight due to glycosylation.

주요 정보

Purity

>90% SDS-PAGE

발현 시스템

HEK 293 cells

Tags

Avi tag C-Terminus Fc tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The GITR:GITRL binding activity was performed in an inhibitor screening assay kit. In a 96-well plate coated with GITRL, ab271528 is incubated, followed by streptavidin-HRP and addition of an HRP substrate. Chemiluminescence produce is measured using a chemiluminescence reader.

Conjugation

Biotin

Excitation/Emission
Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 7.4 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride

storage-buffer

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보완 제품

Primary Antibodies

AB223841

Anti-GITR antibody [EPR20566]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-GITR antibody [EPR20566] (AB223841)

  • 0
  • 0
Assay Kits

AB234571

Human GITR ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human GITR ELISA Kit (AB234571)

  • 0
  • 0
Proteins & Peptides

AB222349

Recombinant Human CD90 / Thy1 protein (His tag C-Terminus)

SDS-PAGE - Recombinant Human CD90 / Thy1 protein (His tag C-Terminus) (AB222349)

  • 0
  • 0
Primary Antibodies

AB270907

Alexa Fluor® 488 Anti-LAG-3 antibody [CAL77]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-LAG-3 antibody [CAL77] (AB270907)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This protein runs at a higher MW by SDS-PAGE due to glycosylation.</p>" } } }
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제품 세부 정보

Enzymatically biotin-labeled using Avi-tag™ technology.
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서열 정보

[{"linker":null,"sequence":"QRPTGGPGCGPGRLLLGTGTDARCCRVHTTRCCRDYPGEECCSEWDCMCVQPEFHCGDPCCTTCRHHPCPPGQGVQSQGKFSFGFQCIDCASGTFSGGHEGHCKPWTDCTQFGFLTVFPGNKTHNAVCVPGSPPAE","proteinLength":"Fragment","predictedMolecularWeight":"43 kDa","actualMolecularWeight":null,"aminoAcidEnd":161,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9Y5U5","tags":[{"tag":"Avi","terminus":"C-Terminus"},{"tag":"Fc","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
보관 정보
Avoid freeze / thaw cycle|Store in the dark
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GITR also known as TNFRSF18 is a 241 amino acid protein with a mass of approximately 27 kDa. It belongs to the tumor necrosis factor receptor superfamily. GITR is highly expressed on activated T cells and regulatory T cells (Tregs). The gene encoding GITR is located on chromosome 1 in humans. High surface expression is also observed in immune tissues such as the thymus spleen and lymph nodes playing an important role in immune responses.
Biological function summary

GITR acts as a costimulatory molecule that enhances T cell activation and proliferation. It does not function as part of a complex but interacts directly with ligand GITRL triggering downstream signaling cascades. This interaction is key in the modulation of immune responses notably in enhancing the activity of effector T cells and regulating Treg functions. Activation of GITR can reduce Treg-mediated suppression leading to enhanced immune responses.

Pathways

The mechanistic role of GITR involves NF-kB and MAPK signaling pathways. Through these GITR impacts immune responses by stimulating the production of cytokines and promoting cell survival and proliferation. GITR signaling intersects with pathways involving proteins such as NF-kB further integrating with the immune system's regulation crescendo.

GITR appears to play a role in autoimmune conditions like rheumatoid arthritis and potential cancer immunotherapy. In autoimmune diseases GITR modulates immune activity influencing the balance between inhibition and activation of T cells. In oncology GITR targeting aims to enhance immune responses against tumors. Through these conditions GITR shows connections to proteins such as CTLA-4 which also contributes to immune regulatory processes.
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일반 정보

기능

Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

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제품 프로토콜

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타겟 정보

Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
See full target information TNFRSF18
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Product promise

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