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AB130280

Recombinant Human GLP-1R protein

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Recombinant Human GLP-1R protein is a Human Fragment protein, in the 24 to 145 aa range, expressed in Escherichia coli, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, WB.
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SDS-PAGE - Recombinant Human GLP-1R protein (AB130280)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GLP-1R protein (AB130280)

14% SDS-PAGE separation of ab130280 :
Lane 1 : M.W. marker – 97, 66, 45, 31, 21, 14 kDa
Lane 2 : reduced and boiled sample, 5μg/lane.
Lane 3 : non-reduced and non-boiled sample, 5μg/lane.

주요 정보

Purity

>90% Densitometry

ab130280 is purified using Ni-NTA chromatography and filtered (0.4 µm).

Endotoxin 레벨

< 1 EU/µg

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

P43220-1

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute at 0.5 mg/mL in 0.1 M acetate buffer, pH 4.0

보관 버퍼

Constituents: 0.41% Sodium acetate

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

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Primary Antibodies

AB218532

Anti-GLP-1R antibody [EPR21819]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (AB218532)

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Proteins & Peptides

AB235719

Recombinant Human Oxyntomodulin (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Oxyntomodulin (GST tag N-Terminus) (AB235719)

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Reagents, Controls & Accessories

AB119211

InstantBlue® Coomassie Protein Stain (ISB1L)

Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (AB119211)

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Proteins & Peptides

AB316439

Recombinant Human BCMA Protein Standard (His tag)

Sandwich ELISA - Recombinant Human BCMA Protein Standard (His tag) (AB316439)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"MKHHHHHHASRPQGATVSLWETVQKWREYRRQCQRSLTEDPPPATDLFCNRTFDEYACWPDGEPGSFVNVSCPWYLPWASSVPQGHVYRFCTAEGLWLQKDNSSLPWRDLSECEESKRGERSSPEEQLLFLY","proteinLength":"Fragment","predictedMolecularWeight":"15.55 kDa","actualMolecularWeight":null,"aminoAcidEnd":145,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P43220","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GLP-1R also known as glucagon-like peptide-1 receptor plays an important role in glucose metabolism. This receptor is a member of the class B family of G protein-coupled receptors (GPCRs) and has a molecular mass of approximately 53 kDa. GLP-1R is expressed in pancreatic beta cells the central nervous system and other peripheral tissues. It binds to GLP-1 a peptide hormone initiating a cascade of intracellular signaling that is essential for its physiological functions.
Biological function summary

GLP-1R serves as an important player in the regulation of insulin secretion. It is not part of a complex but acts as a standalone receptor. When GLP-1 binds to GLP-1R it triggers the activation of adenylyl cyclase increasing cyclic AMP levels and facilitating insulin secretion. This process is glucose-dependent meaning that it enhances insulin release only when blood glucose levels are elevated therefore preventing hypoglycemia.

Pathways

GLP-1R is an integral component of the incretin signaling pathway and the insulin signaling pathway. Through these pathways it influences key metabolic processes. Incretin hormones like GLP-1 improve glucose-dependent insulin secretion involving proteins such as GLP-1R and other incretin-related receptors. Additionally GLP-1R interacts with insulin receptor pathways to modulate glucose uptake in tissues.

GLP-1R has significant implications in type 2 diabetes and obesity. Its role in enhancing insulin secretion makes it a target for therapeutic interventions using GLP-1 analogs like liraglutide. Liraglutide structure mimics natural GLP-1 effectively agonizing GLP-1R to achieve desired metabolic outcomes. Additionally liraglutide GLP-1 analog therapies show promise in obesity management by influencing appetite regulation through GLP-1R pathways in the central nervous system.
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제품 프로토콜

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타겟 정보

G-protein coupled receptor for glucagon-like peptide 1 (GLP-1) (PubMed : 19861722, PubMed : 26308095, PubMed : 27196125, PubMed : 28514449, PubMed : 7517895, PubMed : 8216285, PubMed : 8405712). Ligand binding triggers activation of a signaling cascade that leads to the activation of adenylyl cyclase and increased intracellular cAMP levels (PubMed : 19861722, PubMed : 26308095, PubMed : 27196125, PubMed : 28514449, PubMed : 7517895, PubMed : 8216285, PubMed : 8405712). Plays a role in regulating insulin secretion in response to GLP-1 (By similarity).
See full target information GLP1R

대체 명칭 보기

Glucagon-like peptide 1 receptor, GLP-1 receptor, GLP-1-R, GLP-1R, GLP1R
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

The Journal of clinical investigation 130:2041-2053 PubMed31961827

2020

Cystic fibrosis transmembrane conductance regulator dysfunction in platelets drives lung hyperinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Guadalupe Ortiz-Muñoz,Michelle A Yu,Emma Lefrançais,Beñat Mallavia,Colin Valet,Jennifer J Tian,Serena Ranucci,Kristin M Wang,Zhe Liu,Nicholas Kwaan,Diana Dawson,Mary Ellen Kleinhenz,Fadi T Khasawneh,Peter M Haggie,Alan S Verkman,Mark R Looney
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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