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AB323071

Recombinant Human Glutathione Peroxidase 1 Protein Standard (His tag)

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Recombinant Human Glutathione Peroxidase 1 Protein Standard (His tag) is a Human Full Length protein, expressed in Escherichia coli, with >80%, suitable for sELISA, SDS-PAGE.
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Sandwich ELISA - Recombinant Human Glutathione Peroxidase 1 Protein Standard (His tag) (AB323071)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Glutathione Peroxidase 1 Protein Standard (His tag) (AB323071)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human Glutathione Peroxidase 1 Protein Standard (His tag) (AB323071)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Glutathione Peroxidase 1 Protein Standard (His tag) (AB323071)

SDS-PAGE analysis of ab323071 under reducing conditions for 2ug protein.

주요 정보

Purity

>80% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

P07203-1

Animal free

Yes

Carrier free

No

Species

Human

보관 버퍼

pH: 7.3 - 7.5 Constituents: 12.6% Glycerol (glycerin, glycerine), 2.922% Sodium chloride, 1.742% L-Arginine, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

  • 0
  • 0
Proteins & Peptides

AB80254

Recombinant Human KMT4 / Dot1L protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human KMT4 / Dot1L protein (GST tag N-Terminus) (AB80254)

  • 0
  • 0
Proteins & Peptides

AB70456

Recombinant S. japonicum GST protein (Tag Free)

SDS-PAGE - Recombinant S. japonicum GST protein (AB70456)

  • 4
  • 1
Proteins & Peptides

AB116170

Recombinant Human Cystatin SN/CST1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Cystatin SN/CST1 protein (His tag N-Terminus) (AB116170)

  • 0
  • 0
Proteins & Peptides

AB225662

Recombinant Human MYBPC3 protein (His tag)

SDS-PAGE - Recombinant Human MYBPC3 protein (His tag) (AB225662)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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서열 정보

[{"linker":null,"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"24.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P07203","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification His Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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일반 정보

기능

Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis (PubMed : 11115402, PubMed : 36608588). Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides (PubMed : 11115402, PubMed : 36608588). In platelets catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway (PubMed : 11115402).

서열 유사성

Belongs to the glutathione peroxidase family.

Post-translational modifications

During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.

Subcellular localisation

Mitochondrion

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제품 프로토콜

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타겟 정보

Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis (PubMed : 11115402, PubMed : 36608588). Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides (PubMed : 11115402, PubMed : 36608588). In platelets catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway (PubMed : 11115402).
See full target information Glutathione peroxidase 1

대체 명칭 보기

Glutathione peroxidase 1, GPx-1, GSHPx-1, Cellular glutathione peroxidase, Phospholipid-hydroperoxide glutathione peroxidase GPX1, GPX1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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