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AB109928

Recombinant Human GLYAT/GAT protein (His tag N-Terminus)

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Recombinant Human GLYAT/GAT protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 296 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

ACGNAT, CAT, GAT, GLYAT, Glycine N-acyltransferase, Acyl-CoA:glycine N-acyltransferase, Aralkyl acyl-CoA N-acyltransferase, Aralkyl acyl-CoA:amino acid N-acyltransferase, Benzoyl-coenzyme A:glycine N-acyltransferase, Glycine N-benzoyltransferase, HRP-1(CLP), AAc

2 이미지
SDS-PAGE - Recombinant Human GLYAT/GAT protein (His tag N-Terminus) (AB109928)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GLYAT/GAT protein (His tag N-Terminus) (AB109928)

15% SDS-PAGE showing ab109928 at approximately 36.0 kDa (3µg).

SDS-PAGE - Recombinant Human GLYAT/GAT protein (His tag N-Terminus) (AB109928)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human GLYAT/GAT protein (His tag N-Terminus) (AB109928)

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

주요 정보

Purity

>90% SDS-PAGE

ab109928 is purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

보관 버퍼

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB110413

Total OXPHOS Rodent WB Antibody Cocktail

Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

  • 5
  • 18
Primary Antibodies

AB124773

Anti-Mitofusin 2 antibody [NIAR164]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (AB124773)

  • 4
  • 6
Assay Kits

AB109721

Complex I Enzyme Activity Microplate Assay Kit (Colorimetric)

Functional Studies - Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) (AB109721)

  • 5
  • 4
Assay Kits

AB109911

Complex IV Rodent Enzyme Activity Microplate Assay Kit

Functional Studies - Complex IV Rodent Enzyme Activity Microplate Assay Kit (AB109911)

  • 5
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

This product was previously labelled as GLYAT
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMMLPLQGAQMLQMLEKSLRKSLPASLKVYGTVFHINHGNPFNLKAVVDKWPDFNTVVVCPQEQDMTDDLDHYTNTYQIYSKDPQNCQEFLGSPELINWKQHLQIQSSQPSLNEAIQNLAAIKSFKVKQTQRILYMAAETAKELTPFLLKSKILSPSGGKPKAINQEMFKLSSMDVTHAHLVNKFWHFGGNERSQRFIERCIQTFPTCCLLGPEGTPVCWDLMDQTGEMRMAGTLPEYRLHGLVTYVIYSHAQKLGKLGFPVYSHVDYSNEAMQKMSYTLQHVPIPRSWNQWNCVPL","proteinLength":"Full Length","predictedMolecularWeight":"36 kDa","actualMolecularWeight":null,"aminoAcidEnd":296,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q6IB77","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GLYAT also known as glycine-N-acyltransferase or GAT is an enzyme involved in glycine conjugation. This enzyme has a molecular mass of approximately 33 kDa. GLYAT is mainly expressed in the liver and is found in mitochondria. Its mechanical role involves transferring acyl groups from acyl-CoA to glycine forming acyl-glycine conjugates. These conjugates facilitate the excretion of harmful metabolites from the body contributing to detoxification processes.
Biological function summary

GLYAT functions as part of a detoxification pathway. By conjugating glycine to various acyl-CoA substrates it assists in the removal of xenobiotics and other acyl-containing toxins. This action is particularly important for the prevention of acidosis and maintenance of metabolic homeostasis. The enzyme does not typically form part of a larger complex but works alongside other enzymes involved in amino acid metabolism.

Pathways

GLYAT plays an essential role in the glycine conjugation pathway. This pathway is important for detoxifying benzoic acid and other aromatic carboxylic acids. Related proteins include various CoA synthetases which provide the acyl-CoA substrates needed for GLYAT activity. The glycine conjugation pathway intersects with central metabolic networks like amino acid metabolism linking GLYAT functionally to both catabolic and anabolic processes.

Impaired GLYAT function may relate to metabolic issues like hyperglycinemia and certain cases of congenital metabolic disorders of detoxification. Its activity is also relevant in conditions characterized by an accumulation of organic acids. Dysfunctional GLYAT can lead to toxic metabolite build-up emphasizing its importance in maintaining metabolic health. Disorders connected to GLYAT often involve other enzymes in detoxification pathways such as those enzymes implicated in amino acid degradation.
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일반 정보

기능

Mitochondrial acyltransferase which transfers an acyl group to the N-terminus of glycine and glutamine, although much less efficiently. Can conjugate numerous substrates to form a variety of N-acylglycines, with a preference for benzoyl-CoA over phenylacetyl-CoA as acyl donors. Thereby detoxify xenobiotics, such as benzoic acid or salicylic acid, and endogenous organic acids, such as isovaleric acid.

서열 유사성

Belongs to the glycine N-acyltransferase family.

Subcellular localisation

Mitochondrion

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제품 프로토콜

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타겟 정보

Mitochondrial acyltransferase which transfers an acyl group to the N-terminus of glycine and glutamine, although much less efficiently. Can conjugate numerous substrates to form a variety of N-acylglycines, with a preference for benzoyl-CoA over phenylacetyl-CoA as acyl donors. Thereby detoxify xenobiotics, such as benzoic acid or salicylic acid, and endogenous organic acids, such as isovaleric acid.
See full target information GLYAT
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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